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一氧化碳(CO)是目前影响人类健康的主要空气污染物和空气污染监测指标之一.CO进入人体后的主要存在形式碳氧血红蛋白(HbCO)的生物半衰期在128～409min之间,但动物体内HbCO的消长规律报道很少.为便于用动物研究CO的毒性,我们对大白鼠体内HbCO的生物半衰期进行了实验研究,现将结果报告如下.1 材料与方法1.1 CO来源 以普通蜂窝煤炉作为CO发生源.1.2 动物选择 取健康大白鼠雌雄各4只.体重范围雌性在 240.0～278 og之间,雄性在 310.0～457.5g之间.1.3 实验方法 将大白鼠置室外暴露2h之后,室外CO浓度为3.71mg/m~3,采尾血20μl和40μl分别测定Hb和HbCO含量.然后将其移入容积为38.73m~3的染毒室内染毒30min,染毒室内平均CO浓度为349.25mg/m~3.在停止接触后的0、30、60、90、120min分别采尾血40μl,用CO-I型一氧化碳测定仪测定血样与浓硫酸作用释出的CO量,进而计算CO含量.
Carbon monoxide (CO) is currently one of the main air pollutants and air pollution monitoring indicators affecting human health.COH enters the human body after the main form of carboxyhemoglobin (HbCO) biological half-life of 128 ~ 409min, but in animals HbCO Of the growth and decline of the law rarely reported in order to facilitate the use of animals to study the toxicity of CO, we conducted an experimental study of the biological half-life of HbCO in rats, the results are reported as follows.1 Materials and methods 1.1CO sources to common honeycomb furnace as CO occurs Source.1.2 animal selection Take healthy male and female 4. Body weight range of females between 240.0 ~ 278 og, males between 310.0 ~ 457.5g.1.3 Experimental Methods The rats exposed to outdoor 2h, the outdoor CO concentration 3.71mg / m ~ 3, tail blood 20μl and 40μl were measured Hb and HbCO content.And then moved into a volume of 38.73m ~ 3 in the exposure room exposure 30min, exposure indoor average CO concentration of 349.25mg / m ~ 3. After the contact was stopped at 0, 30, 60, 90, 120min respectively tail blood 40μl, CO-I type carbon monoxide determination of blood samples and concentrated sulfuric acid released CO amount, and then calculate the CO content.