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PFT1/MED25(PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25)在植物中组织发育、成花诱导及胁迫响应等多种进程中起重要调控作用。本研究采用RT-PCR和RACE技术结合的技术克隆了红掌PFT1/MED25基因(Aa PFT1)全长2 457 bp的c DNA序列,推测其含有1个1 974 bp的完整开放阅读框(ORF),编码658个氨基酸。定量RT-PCR分析表明:Aa PFT1在红掌‘热情’各组织中都有表达,其中根的表达量较高;苗期表达量高于成年期;在叶和花的不同组织发育阶段,Aa PFT1在幼嫩组织和成熟组织中表达量较高。Aa PFT1在一年的生长周期内,表现出动态表达变化,但在叶片、苞片、花序中的变化趋势不同。红掌‘卫城’的外源激素处理表明:Aa PFT1能被SA诱导迅速诱导上调,而其他激素处理早期无显著变化。这些结果为进一步解析Aa PFT1基因在红掌中可能的多重作用机制奠定基础。
PFT1 / MED25 (PHYTOCHROME AND FLOWERING TIME1 / MEDIATOR25) plays an important regulatory role in many processes such as tissue development, floral induction and stress response in plants. In this study, we cloned the cDNA sequence of a 2 457 bp full length Aa PFT1 from Anthurium thunbergii by RT-PCR and RACE techniques. It is speculated that it contains a complete 1 974 bp open reading frame (ORF) , Encoding 658 amino acids. Quantitative RT-PCR analysis showed that Aa PFT1 was expressed in all tissues of Anthurium andraeanum, among which the root expression was higher, the seedling stage was higher than that in adulthood. In the stages of leaf and flower development, Aa PFT1 is highly expressed in young and mature tissues. Aa PFT1 showed dynamic expression changes in the growth cycle of one year, but showed different trends in leaf, bracts and inflorescence. The exogenous hormones treatment of Anthurium showed that Aa PFT1 could be rapidly induced up-regulated by SA, while no significant changes were observed in the early stages of other hormone treatments. These results lay the foundation for further analysis of the possible multiple mechanism of action of Aa PFT1 gene in Anthurium andraeanum.