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目的:为明确温度、光照(包括紫外光)对白僵菌加拿大1号菌株菌落、产孢、孢子萌发等的影响.方法:将加1孢子接种到不同光照和温度处理的培养皿中观菌丝生长速率、产孢量、产孢密度及经紫外照射后孢子萌发率的变化.结果:培养14d后,全光照处理菌落直径最小,孢子密度最大;全黑暗处理菌落直径最大,孢子密度最小;前6d黑暗后8d光照处理的产孢量最大.28℃为菌丝生长的最佳温度,14d时菌落直径最大,产孢最多;而19℃对菌丝生长最慢,14d时菌落直径最小,产孢最少,22℃条件为孢子产生的最适宜温度,产孢密度最大.紫外光对孢子活力均有显著影响,随紫外光照时间的延长,孢子萌发率显著降低,照射2h后,孢子完全失活.结论:黑暗处理有利于菌丝生长,光照能促进孢子产生;28℃有利于菌丝生长,22℃有利于孢子产生;紫外光照能显著抑制孢子活力.
Objective: To clarify the effects of temperature, light (including ultraviolet light) on the colony, sporulation and spore germination of Beauveria bassiana strain No. 1 in Canada.Methods: The spores were inoculated into the petri dish with different light and temperature Growth rate, sporulation volume, sporulation density and spore germination rate after UV irradiation were observed.Results: After 14 days of culture, the diameter of the colony was the smallest and the spore density was the highest, After 6 days of darkness, the amount of sporulation reached the maximum after 8 days of dark treatment, the best temperature for mycelial growth was 28 ° C, and the largest diameter and sporulation occurred on the 14th day, while it was the slowest for mycelial growth at 19 ° C and the smallest on the 14th day. Spores were the least, spore density was the highest at 22 ℃, spore density was the highest, UV light had a significant effect on spore vigor, spore germination rate was significantly decreased with the extension of UV light time, spores completely inactivated 2h after irradiation .Conclusion: Dark treatment is conducive to mycelial growth, light can promote spore production; 28 ℃ conducive to mycelial growth, 22 ℃ conducive to spore production; UV light significantly inhibited spore activity.