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目的探讨白细胞介素-6(Interleukin-6,IL-6)基因启动子区-572位点单核苷酸多态性(single nucleotide polymorphism,SNP)与儿童肠道病毒71型(enterovirus 71,EV71)感染疾病严重程度的相关性。方法提取87份EV71重症感染患者和57份无症状隐性感染儿童血液样本基因组DNA,进行PCR扩增及测序,检测IL-6基因启动子区-572位点多态性,利用Logistic回归分析基因型频率。结果 EV71重症感染患者IL-6基因型频率与对照组相比,差异有统计学意义(OR=2.605,95%CI:1.117~6.074,P=0.027)。IL-6多态性与EV71感染严重程度有关(OR=2.411,95%CI:1.236~4.706,P=0.010)。结论 IL-6多态性与中国儿童EV71重症感染可能具有相关性,对确定影响疾病严重程度的宿主遗传因素和增强治疗效果具有重要意义。
Objective To investigate the relationship between single nucleotide polymorphism (SNP) of interleukin-6 (IL-6) gene promoter region at -572 and enterovirus 71 (EV71 ) The severity of the infection. Methods The genomic DNA of 87 EV71 patients and 57 asymptomatic children with latent infection were extracted and amplified by PCR. The polymorphism at -572 locus in IL-6 gene promoter region was detected. Logistic regression was used to analyze the gene Type frequency Results The frequency of IL-6 genotypes in severe EV71 patients was significantly higher than that in control group (OR = 2.605, 95% CI: 1.117-6.074, P = 0.027). IL-6 polymorphism was associated with the severity of EV71 infection (OR = 2.411, 95% CI: 1.236-4.706, P = 0.010). Conclusion IL-6 polymorphism may be related to severe EV71 infection in Chinese children, which is of great significance in determining the genetic factors affecting the severity of the disease and enhancing the therapeutic effect.