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随着单克隆抗体在各种组织中定位研究的广泛开展,免疫过氧化物酶技术已成为常规病理诊断手段之一。但往往由于内源性过氧化物酶的存在而干扰了正常的免疫酶反应。尤其对小肠冰冻组织切片进行免疫组化研究时,过去常用的去除内源酶的方法对此则难以奏效。本文着重介绍一种方法,即能有效地去除背景的非特异染色,又不影响单克隆抗体与各种组织成份中相应抗原的结合,为免疫组织化学研究的顺利进行提供了
With the extensive research on the localization of monoclonal antibodies in various tissues, the immuno-peroxidase technology has become one of the routine pathological diagnosis methods. But often due to the presence of endogenous peroxidase and interfere with the normal immune enzyme reaction. In particular, immunohistochemistry of frozen sections of small intestine was not successful in removing endogenous enzymes in the past. This article focuses on a method that can effectively remove the background of non-specific staining, and does not affect the monoclonal antibody and various tissue components of the corresponding antigen binding for the immunohistochemistry to provide a smooth