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背景:肿瘤的细胞遗传学研究表明,肿瘤染色体的改变具有非随机性,一些肿瘤还存在有特异的染色体异常,从而为癌基因的表达提供了细胞遗传学基础。目的:对人类乳腺癌细胞系Bcap-37和MCF-7进行染色体G显带分析,研究其核型特征和标记染色体。设计:以细胞为观察对象的对照实验。单位:北京大学医学部医学遗传学系。材料:实验于1991-04/1992-05在北京大学医学部医学遗传学系完成。人类乳腺癌细胞系Bcap-37和MCF-7。方法:应用低温同步法与秋水酰胺处理制备染色体标本,对人类乳腺癌细胞系Bcap-37和MCF-7的中期及早中期细胞进行G-显带分析。对每个细胞系计数50～60个分裂相,分析15～16个G-显带核型,包括320条带和500条带左右水平的分裂相。主要观察指标:两个乳腺癌细胞系染色体数目以及结构改变。结果:Bcap-37细胞染色体众数为63,可识别其结构的标记染色体17条;MCF-7细胞染色体众数为56,可识别其结构的标记染色体13条。结论:两个乳腺癌细胞系均有复杂的染色体异常,乳腺癌中染色体结构及数目的异常,它们可能引起肿瘤相关基因DNA序列重排,也可能导致某些染色体DNA丢失,从而在乳腺癌发生发展中起一定作用。
BACKGROUND: Cytogenetical studies of tumors show that tumor chromosomal alterations are nonrandomized and that some tumors have specific chromosomal abnormalities that provide cytogenetics for oncogene expression. OBJECTIVE: To carry out chromosome G banding analysis of human breast cancer cell lines Bcap-37 and MCF-7 to study their karyotype characteristics and marker chromosomes. Design: Controlled experiment with cells as the observation object. Unit: Department of Medical Genetics, Peking University Health Science Center. Materials: The experiment was performed at Department of Medical Genetics, Peking University Health Science Center from April 1991 to May 1992. Human breast cancer cell lines Bcap-37 and MCF-7. Methods: Chromosome specimens were prepared by cryogenic synchronization and colchicine treatment. G-banding analysis was performed on the medium and early metaphase cells of human breast cancer cell lines Bcap-37 and MCF-7. Fifty to sixty cleavage phases were counted for each cell line and fifteen to sixteen G-banding karyotypes were analyzed, including 320 bands and 500 zygaphalitic phases. MAIN OUTCOME MEASURES: Chromosome number and structural changes in two breast cancer cell lines. Results: The chromosome number of Bcap-37 cells was 63, 17 of them could be identified. The number of chromosomes of MCF-7 cells was 56, and 13 of the chromosomes were identified. CONCLUSIONS: Both breast cancer cell lines have complex chromosomal abnormalities, chromosomal structure and number of abnormalities in breast cancer, which may cause DNA sequence rearrangement of tumor-associated genes and may also lead to the loss of some chromosomal DNA, which may result in the occurrence of breast cancer Play a role in development.