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目的探讨不同饮用水对小鼠非特异性免疫的影响。方法将160只3周龄清洁级ICR小鼠随机分为4组,分别为自来水组、纯净水组、矿泉水组、净化水组,每组40只,雌雄各半。各组小鼠自由饮水摄食60 d。选取80只小鼠进行碳廓清实验,称重,测定胸腺、脾脏重量并计算脏器系数;另选80只清洁级ICR小鼠进行小鼠腹腔巨噬细胞吞噬鸡红细胞实验。结果各组小鼠的体重、脾脏系数、胸腺系数、廓清指数及吞噬指数间比较,差异均无统计学意义(P>0.05);但与其他3组比较,净化水组胸腺系数和廓清指数有上升趋势。与自来水组、纯净水组比较,净化水组雄性小鼠的腹腔巨噬细胞吞噬百分率和吞噬指数升高,差异均有统计学意义(P<0.05);净化水组雌性小鼠的腹腔巨噬细胞吞噬百分率和吞噬指数高于自来水组、纯净水组及矿泉水组,差异均有统计学意义(P<0.05)。结论与自来水、纯净水及矿泉水相比,长期饮用净化水在一定程度上可提高小鼠非特异性免疫力。
Objective To investigate the effect of different drinking water on non-specific immunity in mice. Methods 160 3-week-old clean-grade ICR mice were randomly divided into 4 groups: tap water group, pure water group, mineral water group and purified water group, 40 in each group. Each group of mice drinking water for 60 days. Eighty mice were selected for carbon clearance test, weighed, the weight of thymus and spleen were measured, and the organ coefficient was calculated. Another 80 clean-grade ICR mice were used to inoculate mice peritoneal macrophages with chicken erythrocytes. Results There was no significant difference in body weight, spleen coefficient, thymus coefficient, clearance index and phagocytic index between the three groups (P> 0.05). However, compared with the other three groups, the thymus index and clearance index Upward trend. Compared with the tap water group and pure water group, the phagocytic percentage and phagocytic index of peritoneal macrophages in purified water group were significantly increased (P <0.05); the peritoneal macrophage The phagocytic percentage and phagocytic index were higher than those in tap water group, pure water group and mineral water group (P <0.05). Conclusion Compared with tap water, pure water and mineral water, long-term consumption of purified water can improve mice non-specific immunity to a certain extent.