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目的 观察脑通口服液的急性和长期毒性作用。方法 1急性毒性实验 :取健康小白鼠4 0只 ,随机分为两组 (每组 2 0只 ) ,给药组以脑通口服液 10 ml/ kg,对照组给以等量生理盐水灌胃 ,一次性给药观察 7d,求得最大耐受量。另取小白鼠 80只 ,随机等分 8组 ,分别按对数剂量 1次灌胃给药 ,观察 7d,计算半数致死量 ( L D50 )。 2长期毒性实验 :取 Wistar大鼠 80只 ,随机分为大、中、小剂量组和对照组 ,给药组分别以不同浓度剂量的脑通口服液连续灌胃 4 2 d。结果 1急性毒性观察 :两组小鼠 7d内无 1例死亡 ,外观行为及各生理指标差异无显著性 ,显示该药最大耐受量 >10 ml/ kg。计算 LD50 及 L D50 95%平均可信限为 15.4 8± 1.62 ml/ kg,标准误 sx50 为 0 .0 134。 2长期毒性观察 :动物外观、饮食、行为、体重、血象、肝肾功能 ,主要脏器的脏器系数和病理学检查均未发现异常变化。结论 脑通口服液属无毒类药物。
Objective To observe the acute and long-term toxic effects of Naotong oral liquid. Method 1 Acute toxicity test: A total of 40 healthy mice were randomly divided into two groups (20 in each group). The administration group was administered with NaoTong oral solution (10 ml/kg), and the control group was given an equal volume of normal saline. 7d was observed after one-time administration to determine the maximum tolerated dose. Another 80 mice were randomly divided into 8 groups and were given a log dose of 1 gavage, observed for 7 days, and the median lethal dose (LD50) was calculated. 2 Long-term toxicity test: 80 Wistar rats were randomly divided into large, medium and small dose groups and control groups. The administration group was given intragastric administration of different concentrations of Naotong oral liquid for 42 days. Results 1 Observation of acute toxicity: There was no death in 7 days in both groups, and there was no significant difference in appearance behavior and physiological indicators. The maximum tolerable dose was >10 ml/kg. The 95% confidence limit for LD50 and L D50 was calculated to be 15.4 8±1.62 ml/kg, and the standard error sx50 was 0. 0 134. 2 Long-term toxicity observation: Animal appearance, diet, behavior, body weight, hemogram, liver and kidney function, no significant abnormalities were found in organ coefficients and pathological examinations of major organs. Conclusion Naotong oral solution is a non-toxic drug.