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为了揭示春兰品种的遗传多样性和亲缘关系,为春兰种质资源的有效利用和开发提供依据,采用AFLP技术对51个春兰品种进行了遗传多样性分析,经筛选得到了8对条带清晰、多态性高的引物,共扩增出1315条DNA片段,其中多态性条带为1217条,平均1对引物扩增条带164条,多态性带152条,多态性位点频率为92.5%,表明春兰品种具有丰富的遗传多态性。49个品种含有特有带。51个品种间遗传相似系数变化范围为0.501~0.716,聚类分析表明,51个春兰品种共分为5个类群,来自同一地区的品种并没有聚在一起,表明春兰品种的遗传背景混乱。AFLP分子标记技术能有效地分析春兰品种的遗传多样性和亲缘关系。
In order to reveal the genetic diversity and genetic relationship of Chunlan cultivars, we provided the basis for the effective utilization and development of Chunlan germplasm resources. The genetic diversity of 51 Chunlan cultivars was analyzed by AFLP. Eight pairs of bands were identified as clear bands, A total of 1315 DNA fragments were amplified, including 1217 polymorphic bands, an average of 164 pairs of amplified bands with a pair of primers, 152 polymorphic bands, polymorphic loci frequency 92.5%, indicating that Chunlan varieties have rich genetic polymorphism. 49 varieties contain unique bands. The genetic similarity coefficients of 51 cultivars ranged from 0.501 to 0.716. Cluster analysis showed that 51 Chunlan cultivars were divided into five groups, and the cultivars from the same area did not get together, indicating that the genetic background of Chunlan cultivars was confused. AFLP molecular markers can effectively analyze the genetic diversity and genetic relationship of Chunlan cultivars.