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目的对引起一起疑似食物中毒事件中的病原菌分离株进行病原学检测,并通过脉冲场凝胶电泳(PFGE)追溯致病菌的同源性。方法参照GB 4789.7-2013和相关规范对采回样本进行实时荧光定量PCR初筛、分离培养,以及生化鉴定、血清学分型、神奈川试验和药敏试验。使用脉冲场凝胶电泳(PFGE)技术分析致病菌的同源性。结果 14份样品中有5份检出副溶血性弧菌,血清学分型结果为O3:K6型,神奈川试验结果均为阳性。所检出的致病菌株对常见抗生素(阿米卡星、优立新、氨曲南、庆大霉素、环丙沙星、头孢类、呋喃坦啶、复方新诺明、哌拉西林、妥布霉素)敏感,而对氨苄西林耐药。PFGE分析结果提示其中4株样本菌株具有同源性,而另一株与其它菌株在分子水平上紧密相关。结论本事件是由副溶血性弧菌引发的群体性食物中毒,且致病菌来自同一污染源。
Objective To etiologically detect pathogenic bacteria isolates that cause a suspected food poisoning event and to trace the homology of pathogenic bacteria by pulsed-field gel electrophoresis (PFGE). Methods According to GB 4789.7-2013 and related norms, real-time PCR was used to screen, separate and culture biochemical identification, serological typing, Kanagawa test and drug sensitivity test. Pulsed-field gel electrophoresis (PFGE) was used to analyze the homology of pathogenic bacteria. Results Vibrio parahaemolyticus was detected in 5 out of 14 samples. The result of serological typing was O3: K6, and the result of Kanagawa was positive. The detected pathogenic strains of common antibiotics (amikacin, etoposide, aztreonam, gentamicin, ciprofloxacin, cephalosporins, furadantin, cotrimoxazole, piperacillin, Ampicillin) sensitive, but resistant to ampicillin. The results of PFGE suggested that four strains had homology, while the other strain was closely related to other strains at the molecular level. Conclusion This incident is a group of food poisoning caused by Vibrio parahaemolyticus, and pathogens from the same source of pollution.