HPLC法测定红豆杉皮粗提物中紫杉醇的含量

• 作 者: 刘松青 高振同 代青 张春华
• 机 构: 第三军医大学西南医院药剂科,第三军医大学西南医院药剂科,第三军医大学西南医院药剂科,第三军医大学西南医院药剂科 重庆 400038,重庆 400038,重庆 400038,重庆 400038
• 出 处: 重庆中草药研究
• 发表日期: 1999年02期
• 关键词: 粗提物 红豆杉 紫杉醇含量 HPLC 红豆杉皮粗提物 杉皮 日内精密度 外标法 固定相 紫杉烷类化合物

本文建立了测定红豆杉皮粗提物中紫杉醇含量的HPLC法,以C_(18)柱为固定相,以甲醇-乙腈-水-四氢呋喃(15:40:40:5,v/v)为流动相,检测波长254nm,柱温30±1℃,流速1.2ml/min,以峰高外标法定量,得标准曲线:C=0.1098+0.002613×R,r=0.9996。紫杉醇含量为12.61%,加样回收率:102.1%,日内精密度RSD<2%(n=7)。本法操作简便、灵敏度高,专属性强。

In this paper, HPLC method was established for the determination of paclitaxel in crude extracts of Taxus cuspidata. The stationary phase was C 18 column and methanol-acetonitrile-water-tetrahydrofuran (15:40:40:5, v/v) was used as the mobile phase. Phase, detection wavelength 254nm, column temperature 30±1°C, flow rate 1.2ml/min, quantified by peak height external standard method, obtained standard curve: C=0.1098+0.002613×R, r=0.9996. The paclitaxel content was 12.61%, the recovery rate of the added sample was 102.1%, and the intra-day precision RSD was < 2% (n=7). This method is easy to operate, with high sensitivity and strong specificity.