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哈拉西泮(Halazepam,HZ)最近已由美国FDA批准,用于治疗忧虑和紧张。本文作者采用简便、省时的固相提取程序制备血浆样本,以快速、灵敏的HPLC法同时测定血浆中HZ和去甲西泮(NDZ)含量。试剂和标准 HZ、NDZ、安定(DZ)、甲醇、磷酸钾、甘氨酸。提取设备 C_8 Bond-Elut柱,Vac-E1utSPS24提取装置。仪器和色谱条件 HPLC系统由双联活塞、501型正向溶剂释放装置、712型自动进样器、730型程序多波长多道检测器、745 B型电子积分仪组成。10 cm×4.6mm Adsorbosphere C_8柱,粒度3μm。流动相为0.02
Halazepam (HZ) has recently been approved by the U.S. FDA for the treatment of anxiety and stress. The authors used a simple, time-saving method for the preparation of solid-phase extraction of plasma samples, rapid, sensitive HPLC method for simultaneous determination of plasma HZ and nordiazepam (NDZ) content. Reagents and standards HZ, NDZ, stability (DZ), methanol, potassium phosphate, glycine. Extraction equipment C_8 Bond-Elut column, Vac-E1utSPS24 extraction device. Instrumentation and Chromatographic Conditions The HPLC system consists of a dual-piston, a positive 501 solvent release, a Model 712 autosampler, a Model 730 multi-wavelength multichannel detector and a Model 745 B electron integrator. 10 cm × 4.6 mm Adsorbosphere C_8 column with 3 μm particle size. The mobile phase is 0.02