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目的:探讨孕妇外周血中膜联蛋白V(annexin V)的表达及其与子痫发病的关系。方法:选择2009年10月～2011年10月子痫前期孕妇120例为研究对象,包括轻度子痫前期60例(轻度组)和重度子痫前期60例(重度组),另选同期健康孕妇60例为健康对照组。采用蛋白质印迹法测定所有孕妇外周血膜联蛋白V的水平,并对所有孕妇外周血凝血功能进行检测。结果:轻度组、重度组和健康对照组外周血膜联蛋白V的水平分别为(0.80±0.08)、(0.72±0.16)和(1.68±0.26),轻度和重度子痫前期组水平均明显低于健康对照组(P<0.05),但轻度和重度子痫前期组差异无统计学意义(P>0.05)。外周血凝血功能各项指标子痫前期各组与健康对照组比较差异无统计学意义(P>0.05)。结论:子痫前期孕妇外周血膜联蛋白V的表达水平降低,但其表达水平与病情的严重程度无关。
Objective: To investigate the expression of annexin V in peripheral blood of pregnant women and its relationship with the pathogenesis of eclampsia. Methods: A total of 120 pregnant women with preeclampsia from October 2009 to October 2011 were selected as study subjects, including 60 mild preeclampsia (mild group) and 60 severe preeclampsia (severe group) 60 healthy pregnant women as healthy control group. Western blotting was used to detect the level of annexin V in peripheral blood of all pregnant women and the blood coagulation function of peripheral blood in all pregnant women was detected. Results: The levels of annexin V in the mild group, the severe group and the healthy control group were (0.80 ± 0.08), (0.72 ± 0.16) and (1.68 ± 0.26), respectively, and those in the mild and severe preeclampsia groups (P <0.05), but there was no significant difference between mild and severe preeclampsia (P> 0.05). Peripheral blood coagulation indicators of each group preeclampsia and healthy control group, the difference was not statistically significant (P> 0.05). Conclusion: The expression of annexin V in peripheral blood of pregnant women with preeclampsia is decreased, but its expression level has nothing to do with the severity of the disease.