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目的探讨蛋白激酶C(PKC)抑制剂PKC412对大鼠膀胱肿瘤的生长和细胞凋亡的影响。方法培养大鼠膀胱癌T739细胞,建立T739大鼠原位膀胱癌模型1周后分为模型对照(A组)、阿霉素膀胱灌注治疗(B组)、阿霉素联合PKC412 1μmol/L膀胱灌注治疗(C组)和阿霉素联合PKC412 10μmol/L膀胱灌注治疗(D组)。8周后处死大鼠,观察肿瘤大小,测定CD31表达,计算微血管密度(MVD),TUNEL法测定肿瘤细胞凋亡。结果 8周后,B、C、D组的膀胱肿瘤的重量均小于A组,D组大于B、C组(P<0.01)。D组MVD低于B、C组(P<0.01)。A、B、C和D组肿瘤细胞凋亡指数分别为5.74±1.36、11.53±1.68、13.44±1.96和21.64±1.58,D组肿瘤细胞凋亡率高于B、C组(P<0.01)。结论阿霉素联合PKC412对膀胱癌细胞生长和增殖抑制作用大于单用阿霉素。
Objective To investigate the effect of protein kinase C (PKC) inhibitor PKC412 on the growth and apoptosis of bladder tumor in rats. Methods T739 cells were cultured in vitro and divided into model group (A), bladder infusion of doxorubicin (B group), adriamycin combined with PKC412 1μmol / L bladder Perfusion therapy (group C) and doxorubicin combined with PKC412 10 μmol / L intravesical instillation (group D). After 8 weeks, the rats were killed, the tumor size was observed, the expression of CD31 was measured, the microvessel density (MVD) was calculated, and the apoptosis of tumor cells was measured by TUNEL. Results After 8 weeks, the weights of bladder tumor in groups B, C and D were less than those in group A, and those in group D were greater than those in groups B and C (P <0.01). MVD in group D was lower than that in group B and C (P <0.01). The apoptotic index of tumor cells in group A, B, C and D were 5.74 ± 1.36, 11.53 ± 1.68, 13.44 ± 1.96 and 21.64 ± 1.58, respectively. The apoptosis rate of tumor in group D was higher than that in group B and C (P <0.01). Conclusion Doxorubicin combined with PKC412 has a greater inhibitory effect on bladder cancer cell growth and proliferation than adriamycin alone.