论文部分内容阅读
目的:探讨他汀类降脂药阿托伐他汀潜在的降脂外机制。方法:人单核细胞来源的巨噬细胞,加入50mg/L氧化低密度脂蛋白(oxLDL)共培养10d,加或不加入不同浓度的阿托伐他汀(浓度范围0.01~0.5μmol/L);酶谱学分析基质金属蛋白酶-9(MMP-9)的活性;一期凝固法测定组织因子(TF)的促凝活性。结果:阿托伐他汀可抑制单核-巨噬细胞的增殖,呈现一定的剂量依赖关系(P<0.05),加入100μmol/L羟甲戊酸后,这种抑制作用消失;0.1μmol/L的阿托伐他汀可显著抑制单核-巨噬细胞表达MMP-9的活性;阿托伐他汀可呈剂量依赖性抑制TF的促凝活性(P<0.05)。结论:阿托伐他汀不仅可抑制单核-巨噬细胞的增殖,而且可抑制单核巨噬细胞活化下表达的MMP-9的活性以及TF的促凝活性。
Objective: To investigate the underlying lipid-lowering mechanism of atorvastatin, a statin lipid-lowering drug. Methods: Human monocyte - derived macrophages were co - cultured with oxLDL (50mg / L) for 10 days with or without different concentrations of atorvastatin (concentration range 0.01-0.5μmol / L). The activity of matrix metalloproteinase-9 (MMP-9) was analyzed by zymography. The procoagulant activity of tissue factor (TF) was determined by phase I coagulation assay. Results: Atorvastatin could inhibit the proliferation of monocyte-macrophage cells in a dose-dependent manner (P <0.05). After addition of 100 μmol / L mevalonic acid, the inhibition disappeared. At 0.1 μmol / L Atorvastatin significantly inhibited the activity of MMP-9 in monocyte-macrophage cells. Atorvastatin inhibited the pro-coagulant activity of TF in a dose-dependent manner (P <0.05). Conclusion: Atorvastatin can not only inhibit the proliferation of monocyte-macrophage, but also inhibit the activity of MMP-9 expressed by activation of monocyte-macrophage and the procoagulant activity of TF.