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目的探讨丙泊酚对正常成人静脉血中性粒细胞(PMN)的呼吸爆发功能、凋亡、PMN上黏附分子CD18表达、白细胞介素8(IL-8)及肿瘤坏死因子α(TNF-α)释放的影响。方法采集6例健康成人志愿者静脉血分别制备PMN,用RPMI1640培养液制成悬液。每例样本均分入3组:空白对照组(PMN+培养液),溶剂对照组(PMN+脂肪乳注射液),丙泊酚组[PMN+丙泊酚(终浓度5mg·L-1)]。PMN加药培养12h后,流式细胞仪检测PMN发生呼吸爆发的平均荧光强度、CD18的表达量及发生早期凋亡的百分比,ELISA法检测培养上清液TNF-α、IL-8的浓度。结果3组PMN呼吸爆发功能、凋亡率、CD18的表达量均无显著差异(P>0.05)。培养上清液中IL-8和TNF-α的浓度3组间比较差异也无显著意义(P>0.05)。结论丙泊酚及其脂肪乳溶剂对正常成人PMN的呼吸爆发功能、凋亡及黏附分子CD18的表达无明显影响,也不会改变PMN炎症介质TNF-α、IL-8的释放。
Objective To investigate the respiratory burst function of propofol in normal adult human venous blood neutrophils (PMN), the expression of CD18, the expression of adhesion molecule CD18 on PMN, interleukin 8 (IL-8) and tumor necrosis factor alpha Effect of release. Methods 6 healthy adult volunteers were collected venous blood were prepared PMN, RPMI1640 medium suspension was made. Each sample was divided into 3 groups: blank control group (PMN + culture medium), solvent control group (PMN + fat emulsion injection), propofol group [PMN + propofol (final concentration 5 mg · L -1)]. After cultured with PMN for 12 hours, the average fluorescence intensity, the expression of CD18 and the percentage of early apoptosis of PMN were detected by flow cytometry. The concentrations of TNF-α and IL-8 in culture supernatants were detected by ELISA. Results The respiratory burst function, apoptotic rate and CD18 expression of PMN in the three groups showed no significant difference (P> 0.05). The concentration of IL-8 and TNF-α in the culture supernatant also showed no significant difference among the three groups (P> 0.05). Conclusion Propofol and its fat emulsion have no effect on the respiratory burst function, apoptosis and expression of adhesion molecule CD18 in normal adult PMN, and do not change the release of TNF-α and IL-8 in PMN inflammatory mediators.