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目的了解小鼠囊胚体外对人子宫内膜癌细胞RL95-2侵袭力及粘附、运动性的影响作用。方法建立小鼠囊胚与人子宫内膜癌细胞RL95-2共培养模型,分别采用Matrigel人工重建基底膜侵袭实验、MTT法粘附实验和Transwell小室趋化性运动模型,来研究与小鼠囊胚共培养后,人子宫内膜癌细胞RL95-2体外侵袭能力、粘附性和运动性的改变。结果小鼠囊胚与人子宫内膜癌细胞RL95-2共培养后,RL95-2细胞穿过Matrigel人工基底膜的细胞数明显减少(14±5.66vs38.08±13.43,P<0.01),30min和60min的粘附率均降低(P<0.01),穿过Transwell小室聚碳酸脂微孔的细胞数也显著减少(24.69±1.57vs59.19±2.39,P<0.01)。结论小鼠囊胚可降低恶性肿瘤细胞的侵袭能力,及侵袭相关的粘附性和趋化运动性。
Objective To investigate the effect of mouse blastocysts on invasiveness, adhesion and motility of human endometrial carcinoma cell line RL95-2 in vitro. Methods The co-culture model of mouse blastocyst and human endometrial carcinoma cell line RL95-2 was established. Matrigel artificial reconstruction of basement membrane invasion assay, MTT adhesion assay and Transwell chemotaxis model were used to investigate Embryo co-culture, human endometrial cancer cell RL95-2 in vitro invasive ability, adhesion and motility changes. Results After co-cultured with mouse blastocysts and human endometrial carcinoma cell line RL95-2, the number of RL95-2 cells passing through the Matrigel artificial basement membrane was significantly decreased (14 ± 5.66 vs. 38.08 ± 13.43, P <0.01), 30 min And 60min (P <0.01). The number of cells passing through Transwell chamber polycarbonate micropores was also significantly reduced (24.69 ± 1.57 vs 59.19 ± 2.39, P <0.01). Conclusions Mouse blastocysts can reduce the invasiveness of malignant cells and invasion-related adhesion and chemotactic motility.