论文部分内容阅读
建立反相高效液相色谱-二极管阵列检测 (RP-HPLC-DAD) 法同时测定经典名方吴茱萸汤中7个生物碱 (去氢吴茱萸碱、10-羟基吴茱萸次碱、吴茱萸碱、吴茱萸次碱、1-甲基-2-正壬基-4(1H)喹诺酮、吴茱萸卡品碱和二氢吴茱萸卡品碱) 和2个黄酮苷 (异鼠李素-7-O-芸香糖苷和香叶木素-7-O-β-D-吡喃葡萄糖苷)的含量。RP-HPLC分离应用反相C18 色谱柱, 甲醇-1%醋酸水溶液梯度洗脱, 流速为1.0 mL/min, 检测波长为300 nm。上述9个分析物达到基线分离, 标准曲线线性关系良好(r2>0.9941), 日内和日间精密度和准确度符合分析方法学要求, 加样回收率为90.13%-102.48% (RSDs<3.6%)。经方法学验证, 所建立的方法适用于吴茱萸汤物质基础的质量控制。
To establish an RP-HPLC-DAD method for the simultaneous determination of seven alkaloids (evodiamine, 10-hydroxy-rutaecarpine, evodiamine, rutaecarpine 1-methyl-2-n-nonyl-4 (1H) quinolone, Evodiamine, and Dihydro Evodiamine) and two flavonoid glycosides (Isorhamnetin-7-O-rutinoside and geraniol -7-O-β-D-glucopyranoside). RP-HPLC separation using reversed-phase C18 column, methanol-1% aqueous acetic acid gradient elution, flow rate of 1.0 mL / min, detection wavelength of 300 nm. The above nine analytes reached baseline separation with a good linear relationship (r2> 0.9941). The intra-day and inter-day precision and accuracy were in accordance with the analytical methodologies. The recovery rates were 90.13% -102.48% (RSDs <3.6% ). The methodological validation, the established method applies to Evodia Tang quality control of the material basis.