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目的:观察胎儿生长受限(FGR)孕妇外周血及新生儿脐血中内皮祖细胞(EPCs)数量与功能的变化。方法:选择FGR孕妇及新生儿15例和对照组健康孕妇及新生儿15例,密度梯度离心法收集外周血及脐血中单个核细胞(MNCs),接种在人纤维连接蛋白包被的培养板,诱导分化培养9d后收集贴壁细胞进行细胞化学分析。激光共聚焦显微镜下FITC-UEA-Ⅰ和Dil-aclLDL双荧光染色,镜下计数双染细胞。用二苯基四氮唑嗅盐(MTT)法、黏附能力测定检测EPCs的黏附能力、增殖能力。结果:FGR孕妇外周血EPCs数量较对照组孕妇外周血减少,两组差异有统计学意义(P<0.05);FGR新生儿脐血EPCs数量较对照组正常足月新生儿明显减少,两组差异有统计学意义(P<0.05);FGR孕妇及新生儿EPCs黏附和增殖功能也相对下降。结论:EPCs数量及功能的减少可能是FGR的病理发生发展的关键因素。
Objective: To observe the changes of the number and function of endothelial progenitor cells (EPCs) in peripheral blood of pregnant women with fetal growth restriction (FGR) and neonatal cord blood. Methods: Fifteen pregnant women and newborn infants with FGR and 15 healthy pregnant women and newborns from the control group were selected. Mononuclear cells (MNCs) from peripheral blood and umbilical cord blood were collected by density gradient centrifugation and cultured in human fibronectin coated culture plates After culturing for 9 days, adherent cells were collected for cytochemical analysis. Double-stained with FITC-UEA-I and Dil-aclLDL under laser scanning confocal microscope. The adhesion and proliferation of EPCs were detected by diphenyltetrazolium chloride (MTT) assay and adhesion assay. Results: The number of EPCs in peripheral blood of pregnant women with FGR was significantly lower than that of pregnant women in the control group (P <0.05). The number of cord blood EPCs in neonates with FGR was significantly lower than that of normal full-term newborns in control group, (P <0.05). The adhesion and proliferation of EPCs in FGR pregnant women and neonate were also decreased. Conclusion: The decrease of the number and function of EPCs may be the key factor of the pathological development of FGR.