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目的了解天津市腹泻婴幼儿腺病毒的感染情况及其分型特点。方法于2008年4月—2009年4月收集天津市儿童医院住院部病毒性腹泻患儿粪便标本766例,通过PCR法确定阳性标本,将阳性标本进行细胞培养产生细胞病变后,再次PCR扩增确证,并将产物测序,进行基因型别分析。结果 766例标本中腺病毒的检出率为17.62%,其中肠道腺病毒Ad41型占阳性标本的86.67%,Ad1型占13.33%,Ad40型未检出。感染腺病毒患儿的年龄中位数为8.00月龄(0.06～24月龄),全部分布在2岁以下。腺病毒感染全年存在,尤以7月感染最多,其次是2月和9月。结论肠道腺病毒是天津市住院婴幼儿病毒性腹泻的主要病原体之一,2008年4月—2009年4月以Ad41型流行为主。
Objective To understand the infectious status of infantile adenovirus in diarrhea in Tianjin and its typing characteristics. Methods From April 2008 to April 2009, 766 cases of stool samples from children with viral diarrhea in Tianjin Children’s Hospital were collected, positive samples were confirmed by PCR, and positive samples were cultured in cell culture to produce cytopathic effect. Confirm, and the product sequencing, genotype analysis. Results The detection rate of adenovirus in 766 samples was 17.62%. The Ad41 type of enterovirus accounted for 86.67% of the positive samples and the Ad1 type accounted for 13.33%. The Ad40 type was not detected. The median age of children with adenovirus infection was 8.0 months (range, 0.06 to 24 months), all of which were under 2 years of age. Adenovirus infection throughout the year, especially in July the most infected, followed by February and September. Conclusions Intestinal adenovirus is one of the major pathogens of infant diarrhea in infants in Tianjin. From April 2008 to April 2009, predominantly Ad41 was reported.