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目的:观察人参皂苷抗脑细胞缺氧损伤的作用.方法:将12d培龄的海马细胞置于95％N_2+5％CO_2中4—24h,比较人参皂苷组和对照组细胞形态学.存活率及LDH和K~+流出的变化.结果:缺氧24h后,对照细胞存活率从缺氧前92％±4％降至1.0％±2.0％;LDH和K~+漏出量分别由2.3±0.6 U L~(-1)和5.56±0.16 mmol L~(-1)增至36±5 U L~(-1)和8.5±0.7 mmol L~(-1);此时,人参皂苷组细胞存活率为4％±4％;LDH漏出量为30+3 U L~(-1),K~+含量为7.9士0.8 mmol L~(1).与对照组相比,人参皂苷组受损程度明显减轻.结论:人参皂苷具有抗海马细胞缺氧损伤的作用.
OBJECTIVE: To observe the effect of ginsenosides on anti-hypoxic injury of brain cells. METHODS: The 12d cultured hippocampal cells were placed in 95% N 2 + 5% CO 2 for 4 to 24 hours. The morphological and survival rates of ginsenoside and control groups were compared. Changes in LDH and K~+ efflux. Results: After 24 h of hypoxia, the viability of control cells decreased from 92%±4% before hypoxia to 1.0%±2.0%; the leakage of LDH and K~+ was 2.3±0.6 respectively. UL-1 and 5.56±0.16 mmol L-1 increased to 36±5 UL-1 and 8.5±0.7 mmol L-1; this time, the survival rate of ginsenoside group cells 4%±4%; LDH leakage is 30+3 UL~(-1), K++ content is 7.9±0.8 mmol L~(1). Compared with the control group, the damage degree of ginsenoside group is significantly reduced. 4. Conclusion: Ginsenoside has anti - hypoxic injury effect on hippocampal cells.