论文部分内容阅读
目的:检测高迁移率族群蛋白B1(HMGB1)在高氧(60%O2)暴露新生小鼠肺组织损伤模型中的表达水平,探讨HMGB1在支气管肺发育不良发病(Bronchopulmonary dysplasia,BPD)机制中的作用。方法:新生足月C57BL/6小鼠随机分为实验组和对照组,每组10只,制备60%氧浓度致新生鼠BPD模型,应用HE染色、Masson染色、放射性肺泡计数(RAC)、免疫荧光和实时荧光定量-PCR技术,观察生后3天、7天、14天肺组织病理改变,HMGB1的蛋白和mRNA表达水平。结果:氧处理组随时间推移,出现肺泡上皮肿胀,肺泡壁增厚,间质水肿,炎症细胞浸润,胶原样物质产生,肺泡结构紊乱,数量减少,较空白对照组明显发育迟滞。HMGB1蛋白和mRNA在3天氧处理组、空白对照组表达差异无统计学意义(P>0.05),7天、14天氧处理组表达均强于相应空白对照组(P<0.05)。结论:在60%浓度氧暴露所致BPD中,HMGB1表达增加;BPD的病理过程可能与HMGB1表达增加有关。
AIM: To investigate the expression of HMGB1 in lung tissue of neonatal mice exposed to hyperoxia (60% O2) and to explore the role of HMGB1 in pathogenesis of bronchopulmonary dysplasia (BPD) effect. Methods: Newborn term C57BL / 6 mice were randomly divided into experimental group and control group, with 10 mice in each group. The model of neonatal rat model with 60% oxygen concentration was established. HE staining, Masson staining, radioactive alveolar count (RAC) Fluorescence and real-time fluorescence quantitative-PCR technique were used to observe the histopathological changes of lung tissue and the expression of HMGB1 protein and mRNA at 3 days, 7 days and 14 days after birth. Results: Compared with control group, the development of alveolar epithelial swelling, alveolar wall thickening, interstitial edema, infiltration of inflammatory cells, production of collagen-like substance, alveolar structure disorder and number decreased with the passage of time. The expressions of HMGB1 protein and mRNA in 3-day oxygen-treated group and blank control group showed no significant difference (P> 0.05). The expression of HMGB1 protein in 7-day and 14-day oxygen-treated group was stronger than that in blank control group (P <0.05). CONCLUSION: HMGB1 expression is increased in BPD induced by 60% oxygen exposure. The pathological process of BPD may be related to the increased expression of HMGB1.