论文部分内容阅读
目的:探讨肺癌组织中K-ras蛋白与Bcl-2蛋白的表达意义及其相关性。方法:选取2005-01-01-2012-06-30在苏州市立医院本部手术切除并经病理确诊的99例肺癌石蜡标本,应用组织芯片和免疫组化法观察K-ras及Bcl-2蛋白在肺癌组织中的表达,分析其表达相关性及与患者临床因素的关系。结果:K-ras和Bcl-2蛋白在肺癌组织中的表达阳性率分别为25.3%(25/99)和26.3%(26/99)。K-ras蛋白的表达与性别、病理类型及分化程度差异有统计学意义,P<0.05;与年龄、有无淋巴结转移及TNM分期等因素差异无统计学意义,P>0.05。Bcl-2蛋白的表达与年龄、性别、病理类型、分化程度、有无淋巴结转移及TNM分期等因素之间差异均无统计学意义,P>0.05。K-ras与Bcl-2蛋白表达呈显著正相关,rs=0.657,P<0.01。结论:K-ras与Bcl-2蛋白可能参与肺癌的发生发展,并且存在相互促进作用。
Objective: To investigate the expression of K-ras protein and Bcl-2 protein in lung cancer and its correlation. Methods: Totally 99 paraffin-embedded specimens of lung cancer were collected from January 2005 to January 01,2012 in our hospital. The K-ras and Bcl-2 proteins were detected by tissue microarray and immunohistochemistry Lung cancer tissue expression, analysis of the correlation between expression and clinical factors in patients with. Results: The positive rates of K-ras and Bcl-2 in lung cancer were 25.3% (25/99) and 26.3% (26/99), respectively. There was significant difference in the expression of K-ras protein between the two groups (P <0.05). There was no significant difference between age, lymph node metastasis and TNM stage (P> 0.05). There was no significant difference in the expression of Bcl-2 protein between age, sex, pathological type, degree of differentiation, lymph node metastasis and TNM stage (P> 0.05). There was a significant positive correlation between K-ras and Bcl-2 protein expression (rs = 0.657, P <0.01). Conclusion: K-ras and Bcl-2 protein may be involved in the development of lung cancer, and there is mutual promotion.