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运用纯培养法和基于16S rRNA基因序列的系统发育分析贵州云台山白云岩表层土可培养细菌的多样性.稀释涂布平板分离共获得131株细菌,限制性内切酶HhaⅠ和HaeⅢ对扩增的16S rRNA基因片段进行酶切分型,根据ARDRA酶切图谱划分为40个操作分类单元.16S rRNA基因序列测定和系统发育分析结果显示,这些菌株隶属于包括厚壁菌门(Firmicutes,64.89%)、β-变形菌纲(β-Proterbacteria,3.82%)、γ-变形菌纲(γ-Proterbacteria,17.56%)、放线细菌门(Actinobacteria,11.45%)和拟杆菌门(Bacteroidetes,3.82%)等5大类群,共13个属.优势菌群为芽胞杆菌属(Bacillus,53.44%),亚优势菌群为寡养单胞菌属(Stenotrophomonas,9.16%)、假单胞菌属(Pseudomonas,8.40%)和微小杆菌属(Exiguobacterium,8.40%).15.00%的有效序列与Gene Bank已知序列相似性小于等于97%,可能为潜在新类群.研究表明,云台山白云岩喀斯特地区不仅含有较为丰富的细菌物种多样性,且潜藏着许多新的微生物资源.
Phylogenetic analysis based on 16S rRNA gene sequence and pure culture method was used to analyze the diversity of culturable bacteria in the topsoil of Yuntaishan dolomite in Guizhou Province.A total of 131 strains of bacteria were obtained by dilution coating and the restriction endonucleases HhaⅠand HaeⅢ were used for amplification 16S rRNA gene fragments were classified into 40 operating taxa according to the ARDRA restriction map.The results of sequence analysis and phylogenetic analysis of 16S rRNA gene showed that these strains belonged to Firmicutes including 64.89% , Β-Proterbacteria (3.82%), γ-Proterbacteria (17.56%), Actinobacteria (11.45%) and Bacteroidetes (3.82%), respectively. , And a total of 13 genera.The dominant bacterium was Bacillus (53.44%), the subdominant flora was Stenotrophomonas (9.16%), Pseudomonas 8.40%) and Exiguobacterium (8.40%). The similarity of 15.00% effective sequence with the known sequence of Gene Bank is less than or equal to 97%, which may be a potential new taxon.Research shows that the karst area of Yuntai Mountain dolomite not only contains more Abundant bacterial species diversity, And lurk many new microbial resources.