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弹性蛋白是构成结缔组织的重要组成部分,本文进一步证实尽管弹性蛋白的重量不及皮肤重量的10％,但皮肤经高压蒸煮处理以消除胶元及其它基质成分后,可以显示出大量的弹力纤维,与用弹力纤维特殊染色后的常规石腊切片完全不同,经处理后的石腊切片中全部真皮布满弹力纤维(图1、2),因此在常规切片中所描述“片断的”弹力纤维乃为一种误解,它反映了错综复杂交织成网络样弹力纤维中的一小段,表明:(1)在常规切片中的弹力纤维很大一部分被胶元纤维所掩蔽;(2)因光镜及透射电镜切片菲薄,不能详尽观察弹力纤维结构及其空间关系所致。本文用三种方法进行了比较,表明Tsuji氏法简易恒定,图
Elastin is an important part of the formation of connective tissue. This paper further confirms that although the weight of elastin is less than 10% of the weight of the skin, the skin can be exposed to a large amount of elastic fibers after being autoclaved to eliminate the collagen and other matrix components. In contrast to conventional paraffin sections specially dyed with spandex, all the dermis in the treated paraffin section is covered with elastic fibers (Figures 1 and 2), so the “fragmented” elastic fibers described in conventional sections are It is a misunderstanding that reflects a short segment of intricately interlaced network-like elastic fibers, indicating that: (1) a substantial portion of the elastic fibers in conventional sections are masked by the gelled fiber; (2) due to light and transmission SEM thin sections, can not be a detailed observation of the structure of the elastic fibers and their spatial relationship. In this paper, three methods are compared, indicating that Tsuji’s method is simple and constant, the figure