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为了防止南芥菜花叶病毒(Arabis mosaic virus,ArMV)传入我国,采用血清学、分子生物学、电镜观察及生物学接种等方法对从荷兰进口的郁金香种苗进行了ArMV检测。结果表明:ArMV血清学反应为强阳性;RT-PCR反应扩增出370bp的特异性目标条带;RT-PCR产物与已报道的3个ArMV部分外壳蛋白基因的核苷酸序列同源性为91.00%～93.24%,氨基酸序列同源性为99%～100%;免疫电镜观察到叶片病汁液中含有直径约30nm的球状病毒粒体;该病毒在黄花烟、白肋烟、矮牵牛和昆诺藜等鉴别寄主上引起坏死和褪绿等典型症状,经DAS-ELISA检测,这些接种寄主均呈ArMV血清学阳性反应。故将该病毒鉴定为南芥菜花叶病毒。
To prevent the introduction of Arabidopsis mosaic virus (ArMV) into China, ArMV detection of tulip seedlings imported from the Netherlands was carried out by using serological methods, molecular biology, electron microscopy and biological inoculation methods. The results showed that the serological response of ArMV was strongly positive. The specific target band of 370bp was amplified by RT-PCR. The nucleotide sequence homology between the RT-PCR product and the reported three ArMV partial coat protein genes was 91.00% -93.24%, and the amino acid sequence homology was 99% -100%. Immunoelectron microscopy showed that the leaf juice contained globular virosomes with a diameter of about 30 nm. The virus was expressed in the leaves of yellow tobacco, burley tobacco, petunia Quinoa and other identified on the host caused by necrosis and chlorotic and other typical symptoms, by DAS-ELISA test, the host were vaccinated ArMV seropositive. Therefore, the virus identified as Arabidopsis mosaic virus.