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目的:研究比较异种煅烧骨和Bio-oss骨分别与富血小板纤维蛋白(platdet-rich fibrin,PRF)联合,及其各自单独使用对牙周骨缺损修复的效果。方法:制备煅烧骨,PRF凝胶及其复合物。选54只新西兰大白兔,制备牙周组织缺损模型,随机分成空白对照组、PRF凝胶组、煅烧骨组、Bio-oss骨组、煅烧骨/PRF凝胶组、Bio-oss骨/PRF凝胶组,将这些材料植入牙周缺损区,每组9只兔子。术后4周、8周和l2周处死动物,获取完整标本,观察影像学和组织学。行组织学染色检测,测量新生牙周组织的高度。结果:X线结果表明:4周、8周和l2周煅烧骨/PRF组和Bio-oss/PRF组平均密度均明显高于对照组且缺损处骨组织连续。组织学观察:实验组均在牙根面可见新生牙骨质样组织、牙周膜和牙槽骨样组织的形成,随着时间的推移逐渐成熟、增多。12周时,煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶较空白对照组明显提高,较其他3组差异有统计学意义(P<0.05)。结论:煅烧骨/PRF凝胶、Bio-oss骨/PRF凝胶复合物可明显促进牙周组织的再生。
OBJECTIVE: To compare the effects of different calcined bone and Bio-oss bone with PRF and their respective effects on the repair of periodontal bone defects. Methods: Calcined bone, PRF gel and their complexes were prepared. Fifty-four New Zealand white rabbits were selected to establish the model of periodontal defect. They were randomly divided into blank control group, PRF gel group, calcined bone group, Bio-oss bone group, calcined bone / PRF gel group and Bio-oss bone / Glue groups were implanted with these materials in the periodontal defect area, 9 rabbits in each group. Animals were sacrificed at 4 weeks, 8 weeks and 12 weeks after operation, and the complete specimens were obtained for imaging and histology. Histological staining was performed to measure the height of nascent periodontal tissue. Results: The X-ray results showed that the average density of calcined bone / PRF group and Bio-oss / PRF group at 4, 8 and 12 weeks were significantly higher than that of the control group and the bone tissue was continuous at the defect site. Histological observation: The experimental group showed that the formation of nascent cementum-like tissue, periodontal ligament and alveolar bone-like tissue gradually became mature and increased with the passage of time. At 12 weeks, the calcined bone / PRF gel and Bio-oss bone / PRF gel were significantly increased compared with the blank control group, which was significantly different from the other three groups (P <0.05). Conclusion: Calcined bone / PRF gel and Bio-oss bone / PRF gel composite can significantly promote the regeneration of periodontal tissue.