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[目的]采用新型直链淀粉-三(5-氯-2-甲基苯基氨基甲酸酯)涂敷型手性固定相在反相色谱条件下拆分烯唑醇对映体。[方法]利用高效液相色谱-手性固定相法考察流动相组成及温度对手性拆分的影响,对烯唑醇对映体进行了旋光测定。[结果]烯唑醇在直链淀粉-三(5-氯-2-甲基苯基氨基甲酸酯)手性固定相上为(-)-对映体先出峰;烯唑醇对映体在所考察的流动相组成(乙腈含量100%~30%)及温度(5~45℃)范围内均未能达到基线分离。[结论](-)-(R)-烯唑醇先于其对映体被洗脱;实验烯唑醇对映体分离的最佳流动相配比为乙腈-水(体积比30∶70),最佳分离温度为25℃;基于线性Van’t Hoff曲线的热力学参数证明对映体的分离受焓影响。
[Objective] The aim was to resolve the enalacon alcohol enantiomer under reversed-phase chromatography using a novel amylose-tris (5-chloro-2-methylphenylcarbamate) coated chiral stationary phase. [Method] The enantioselectivities of diniconazole were determined by high performance liquid chromatography-chiral stationary phase method and the effects of mobile phase composition and temperature on chiral resolution were studied. [Result] Diniconazole was the (-) - enantiomer first peak at the amylose-tris (5-chloro-2-methylphenylcarbamate) chiral stationary phase. The body did not achieve baseline separation within the investigated mobile phase composition (acetonitrile 100% to 30%) and temperature (5 to 45 ° C). [Conclusion] The eluent of (-) - (R) -conazole was eluted prior to its enantiomer. The optimum mobile phase enantiomer separation of enoxaphanol was acetonitrile-water (30:70) The optimal separation temperature is 25 ℃. The thermodynamic parameters based on the linear Van’t Hoff curve prove that the separation of enantiomers is affected by the enthalpy.