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目的 探讨食蟹猴疟原虫感染恒河猴后原虫密度消长和抗体滴度变化及相互关系。 方法 食蟹猴疟原虫实验感染恒河猴后 ,在 7年多的时间内 ,定期进行病原学检测和疟疾荧光抗体检测 ,记录分析原虫密度消长和抗体滴度变化等数据。 结果 实验恒河猴接种食蟹猴疟原虫后 ,最早 3d可检出原虫 ,12～ 19d可达到原虫血症高峰期 ,2 483d(时间最长者 )时还可检出原虫 ;荧光抗体试验显示感染第 8d时可检出阳性 ( 1∶ 2 0 ) ,15 8～ 181d达到高峰滴度 ( 1∶ 6 40～ 1∶ 2 5 6 0 ) ,持续 6 0～ 85 d后抗体滴度逐渐下降 ,到实验结束时各猴抗体滴度还保持在 1∶ 40～ 1∶ 16 0。 结论 食蟹猴疟原虫是一种比较理想的实验虫种 ,用它在疟疾研究和抗疟工作中适时的替代间日疟原虫能获得较好的效果。
Objective To investigate the variation and correlation of protozoa density and antibody titer of Plasmodium macaque in macaque infection. Methods After macaque infection with cynomolgus macaques, rhesus macaques were infected with rhesus macaques for more than seven years. Fluorescent antibodies were detected regularly and the changes of protozoa density and antibody titers were recorded. Results After the rhesus monkeys inoculated with malaria cercopithecidae, the protozoa could be detected in the first 3d days, the protozoosis could be reached at 12 ~ 19d, and the protozoa could be detected at 2483 days (the longest time). Fluorescent antibody test showed The positive titer (1: 2 0) was detected on the 8th day of infection and reached the peak titer (1:640 ~ 1:2556) on the 15th ~ 181th day after infection. The titer of antibody decreased gradually after 60 ~ 85 days, At the end of the experiment, the antibody titer of each monkey remained at 1:40 ~ 1:16 0. Conclusion The malaria cynomolgus is a kind of ideal experimental insects, which can be used to get better effect in malaria research and malaria malaria replacement.