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本文具体描述了从人体血浆中大量分离和纯化抗凝血酶Ⅲ(ATⅢ)的方法。采用的原材料Ⅳ-1是Cohn分部分离法分离血浆的副产品,也是制备α_1蛋白酶抑制剂浓缩物的原材料。制备流程主要分为两步:第一步是糊状的Ⅳ-1通过肝素亲和层析柱与其它蛋白分离,然后在0.5M枸橼酸盐溶液中加热到60℃10小时,除去乙型肝炎病毒和获得性免疫缺陷综合征病毒。因仅此一步纯化的浓缩物加热后常含有大量变性蛋白,故需
This article specifically describes a method for the extensive isolation and purification of antithrombin III (AT III) from human plasma. The raw material IV-1 is a by-product of the Cohn fractionation method for the separation of plasma and the starting material for the preparation of α_1 protease inhibitor concentrates. The preparation process is mainly divided into two steps: the first step is the pasty Ⅳ-1 separated from other proteins by heparin affinity chromatography, and then heated to 60 ° C. for 10 hours in 0.5 M citrate solution to remove type B Hepatitis virus and Acquired Immunodeficiency Syndrome virus. Because only one step purification of the concentrate often contains a large number of denatured protein, it is necessary