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目的探讨α-硫辛酸(α-liplic acid,α-LA)对过氧化氢(H2O2)氧化损伤的大鼠肝细胞及糖代谢酶的影响。方法分别将50,100,200μmol/Lα-LA作用于大鼠肝细胞24 h后,用H2O20.2 mol/L损伤1 h,分别检测细胞活力、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)、细胞中葡萄糖激酶(GK)、糖原合成酶激酶-3(GSK-3)的含量。结果α-LA各剂量组GSH-Px活力与SOD活性分别为141.552~208.171和10.852~13.151U/(mg.prot),均高于H2O2损伤组(P<0.05)。α-LA各剂量组MDA含量为12.064~14.142 nmol/(mg.prot),明显低于H2O2损伤组(P<0.05)。α-LA各剂量组葡萄糖激酶(GK)含量为0.928~1.020 mg/L,明显高于H2O2损伤组(P<0.05),不同剂量α-LA组GSK-3含量与H2O2损伤组比较差异无统计学意义(P<0.05)。结论α-LA对H2O2所致大鼠肝细胞氧化损伤具有一定的保护作用,且提高葡萄糖激酶含量。
Objective To investigate the effects of α-lipoic acid (α-LAI) on rat hepatocytes and glucose metabolism enzymes induced by hydrogen peroxide (H2O2) oxidative damage. Methods After treated with 50, 100 and 200μmol / L α-LA for 24 h, the cells were stained with H2020.2 mol / L for 1 h. The cell viability, superoxide dismutase (SOD), glutathione peroxidation (GSH-Px) activity and malondialdehyde (MDA), the content of glucose kinase (GK) and glycogen synthase kinase-3 (GSK-3) in cells. Results The activities of GSH-Px and SOD in α-LA groups were 141.552 ~ 208.171 and 10.852 ~ 13.151U / (mg.prot) respectively, both higher than those in H2O2-injured group (P <0.05). The content of MDA in α-LA dose group was 12.064-14.1442mmol / (mg.prot), which was significantly lower than that of H2O2 injury group (P <0.05). The content of glucokinase (GK) in α-LA dose group was 0.928-1.020 mg / L, which was significantly higher than that in H2O2-injured group (P <0.05). There was no statistical difference in the content of GSK-3 between different doses of α-LA group and H2O2 injury group Significance (P <0.05). Conclusion α-LA can protect rat hepatocytes from H2O2-induced oxidative damage and increase glucokinase levels.