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目的研究中草药注射液痰热清对呼吸道合胞病毒(RSV、Long株)的抑制作用。方法以病毒唑为阳性对照药,采用细胞培养技术,观察不同药物浓度及不同给药方式下RSV攻击后各组Hep-2细胞的病变效应(CPE),在此基础上采用MTT比色法,测定各组细胞的病毒抑制率。以CPE法计算药物的半数中毒浓度TC50,分别以CPE法及MTT法计算药物的半数有效浓度EC50及治疗指数TI,比较不同药物浓度及不同给药方式下热毒宁对呼吸道合胞病毒(RSV)的抑制作用效果。结果痰热清注射液半数中毒浓度TC50为3.972g/L,预防给药方式、细胞外直接灭活及治疗给药方式均有抗RSV作用,其抗RSV的半数有效浓度(EC50)分别为0.428/1.160/1.189g/L,治疗指数TI分别是9.28、3.42和3.34,痰热清对RSV的抑制作用存在着明显的量效反应关系。结论痰热清对RSV有直接灭活作用,对RSV侵入Hep-2细胞有阻断作用,对RSV在Hep-2细胞内增殖有抑制作用,相同浓度下,以预防作用更显著。
Objective To study the inhibitory effect of Chinese herbal medicine Tanreqing on respiratory syncytial virus (RSV, Long strain). Methods Ribavirin was used as a positive control drug. The cell culture technique was used to observe the pathogenic effects (CPE) of Hep-2 cells in different groups after different doses of RSV challenge. MTT colorimetric assay, The virus inhibition rate of each group was measured. The median lethal dose (TC50) of the drug was calculated by CPE method. The median effective concentration (EC50) and therapeutic index (TI) of the drug were calculated by the CPE method and the MTT method respectively. The effect of rebaudiosine on respiratory syncytial virus ) Inhibitory effect. Results The median lethal dose of Tanreqing Injection was 3.972g / L, and the anti-RSV effect of the mode of administration, the direct deactivation of extracellular matrix and the mode of administration of anti-RSV were all effective. The median effective concentrations (EC50) of RSV were 0.428 /1.160/1.189g / L, therapeutic index TI were 9.28,3.42 and 3.34, Tanreqing RSV inhibition there is a significant dose-response relationship. Conclusions Tanreqing can inactivate RSV directly and block RSV invasion into Hep-2 cells, which can inhibit the proliferation of RSV in Hep-2 cells. In the same concentration, the preventive effect is more significant.