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本研究观察了生长激素释放肽(GHRP)的应用对心衰动物胆碱能神经和肾上腺素能神经支配的影响。Wistar大鼠50只,随机分为:假手术组10只;GHRP组30只;模型组10只。心衰模型成功后4周,Karnovsky-Roots法及免疫组织化学方法分别显示心肌胆碱能神经纤维及肾上腺素能免疫阳性纤维,应用多功能真彩色病理图像分析系统分析两种神经纤维密度。结果显示,模型组大鼠心肌中胆碱能神经纤维和肾上腺素能神经纤维密度明显低于假手术组(P<0.01);GHRP组大鼠心肌中两种神经纤维密度较模型组明显增高(P<0.01),但略低于假手术组神经纤维密度(P<0.05)。上述研究表明GHRP可延缓大鼠心衰后的去神经支配,改善神经重构。
This study looked at the effects of ghrelin on the cholinergic and adrenergic innervation in patients with heart failure. Fifty Wistar rats were randomly divided into sham operation group (n = 10), GHRP group (n = 30) and model group (n = 10). Karnovsky-Roots method and immunohistochemical method were used to display the cardiac cholinergic nerve fibers and adrenergic immunoreactive fibers, respectively, four weeks after the heart failure model was successfully treated. The two kinds of nerve fiber density were analyzed by a multifunctional true color histopathological analysis system. The results showed that the density of cholinergic nerve fibers and adrenergic nerve fibers in the model group was significantly lower than that in the sham operation group (P <0.01); the density of the two nerve fibers in the model group was significantly higher than that of the model group P <0.01), but slightly lower than that of the sham group (P <0.05). The above study shows that GHRP can delay denervation after heart failure in rats and improve neural remodeling.