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目的:研究耐药性颞叶癫痫患者脑组织中ULBP2的表达,探讨其在耐药性颞叶癫痫发病中的意义。方法:从重庆医科大学附属第一医院神经内科建立的耐药性癫痫患者脑组织库中随机抽取42例耐药性颞叶癫痫患者术后脑组织,用基因芯片检测ULBP2 cDNA的表达,用免疫荧光、免疫印记法(Western blot)检测ULBP2的蛋白表达产物,并与12例对照组进行比较。结果:耐药性颞叶癫痫患者颞叶脑组织中编码ULBP2的基因出现高表达;ULBP2蛋白在耐药性颞叶癫痫患者颞叶脑组织中的表达量与对照组相同部位比较明显增高。结论:耐药性颞叶癫痫患者颞叶脑组织中ULBP2基因及蛋白产物表达增高可能与耐药性颞叶癫痫发病中的免疫异常机制有重要关联,甚至可能是关键因素。它很可能为耐药性颞叶癫痫的免疫治疗提供新的靶点。
Objective: To study the expression of ULBP2 in the brain tissue of patients with drug-resistant temporal lobe epilepsy and to explore its significance in the pathogenesis of drug-resistant temporal lobe epilepsy. Methods: Twenty-two patients with drug-resistant temporal lobe epilepsy were randomly selected from brain tissue bank of patients with drug-resistant epilepsy established in the First Affiliated Hospital of Chongqing Medical University. The expression of ULBP2 cDNA was detected by gene chip, Fluorescence and Western blot were used to detect the protein expression of ULBP2, and compared with 12 control groups. Results: The gene encoding ULBP2 in temporal lobe brain tissue was highly expressed in drug-resistant temporal lobe epilepsy patients. The expression level of ULBP2 protein in temporal lobe brain tissue in patients with drug-resistant temporal lobe epilepsy was significantly higher than that in control group. CONCLUSION: The increased expression of ULBP2 gene and protein in the temporal lobe brain tissue in patients with drug-resistant temporal lobe epilepsy may play an important role in the mechanism of immune abnormality in the development of drug-resistant temporal lobe epilepsy and may even be the key factor. It is likely to provide a new target for the immunotherapy of resistant temporal lobe epilepsy.