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目的观察猪骨骼肌缺血预适应对心肌缺血再灌注后坏死面积及心肌MMP-2、MMP-9及TIMP-1的影响。方法10只小型猪被随机分为缺血再灌注(I/R)组和远端预适应(RP)组。采用非开胸法建立心脏I/R模型,通过球囊堵塞左股动脉造成骨骼肌短暂缺血。以三硝基四氮唑红确定心肌梗死范围;以免疫组化和RT-PCR法测I/R心肌中MMP-2、MMP-9和TIMP-1表达及骨骼肌RP对此的影响。结果与I/R组相比,骨骼肌RP明显减少心肌梗死范围。在缺血区,RP明显降低MMP-2和MMP-9的表达,增加TIMP-1表达(P<0.05)。且RP明显减弱MMP-2和MMP-9 mRNA的表达,增强TIMP-1的mRNA表达(P<0.05)。结论骨骼肌缺血预适应心肌不仅可减少心肌坏死范围,还可能对心肌间质产生保护作用。
Objective To observe the effect of skeletal muscle ischemic preconditioning on myocardial necrosis and MMP-2, MMP-9 and TIMP-1 after myocardial ischemia / reperfusion in rats. Methods Ten miniature pigs were randomly divided into ischemia / reperfusion (I / R) group and distal preconditioning (RP) group. Cardiac I / R model was established by non-thoracotomy and transient ischemia of skeletal muscle was induced by occlusion of the left femoral artery. The extent of myocardial infarction was determined by MTT. The expression of MMP-2, MMP-9 and TIMP-1 in I / R myocardium and the effect of skeletal muscle RP on them were detected by immunohistochemistry and RT-PCR. Results Compared with I / R group, skeletal muscle RP significantly reduced the infarct size. In ischemic area, RP significantly decreased the expression of MMP-2 and MMP-9 and increased the expression of TIMP-1 (P <0.05). And RP significantly decreased the expression of MMP-2 and MMP-9 mRNA and increased the mRNA expression of TIMP-1 (P <0.05). Conclusion Skeletal muscle ischemic preconditioning can not only reduce the extent of myocardial necrosis, but also may have a protective effect on myocardial interstitium.