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目的构建含CD／UPRT双自杀基因的重组腺病毒Ad-CD/UPRT,观察其对人肺腺癌细胞的杀伤作用。方法用0-100感染复数的重组腺病毒转染肺腺癌A549细胞后加入(0-1 000)mg／L的5-氟胞嘧啶(5-FC),MTT法检测杀伤效率;将转基因细胞与未转基因细胞按不同比例混合,观察旁观者效应。结果 Ad-CD／UPRT转染A549后对细胞生长有显著的抑制作用,细胞杀伤效率随腺病毒滴度及5-FC浓度的增加而增强;转基因细胞占混合细胞10％以上,即可观察到明显的旁观者效应。结论 Ad-CD／UPRT联合5-FC对肺腺癌A549细胞有显著杀伤作用。
Objective To construct recombinant adenovirus Ad-CD / UPRT containing double suicide genes of CD / UPRT and observe the killing effect on human lung adenocarcinoma cells. Methods A549 cells were transfected with a number of recombinant adenoviruses (0-100) and then treated with 5-fluorouracil (5-FC) (0-1 000) mg / L. MTT assay was used to determine the cytotoxicity Mixed with non-transgenic cells in different proportions to observe bystander effect. Results Ad-CD / UPRT transfected A549 cells significantly inhibited the growth of cell killing efficiency with the adenovirus titer and 5-FC concentration increased; transgenic cells accounted for more than 10% of mixed cells can be observed Obvious bystander effect. Conclusion Ad-CD / UPRT combined with 5-FC has a significant killing effect on lung adenocarcinoma A549 cells.