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目的:探讨补充外源性辅酶Q10(CoQ10)及递增负荷运动训练对一次力竭运动大鼠骨骼肌线粒体呼吸链酶复合体Ⅰ、Ⅱ和Ⅲ活性的影响。方法:36只2月龄健康雄性Wister大鼠随机分为4组(n=9):对照组(NC)、补充CoQ10组(QC)、训练组(NE)和补充CoQ10+训练组(QE)。喂养及训练时间共7周。QC组和QE组每天按2mg/100g体重剂量灌胃补充CoQ10一次。NC组和QC组不进行运动训练,NE组和QE组进行递增负荷水平跑台运动训练。7周后各组均进行一次力竭运动,运动后即刻断头处死,迅速取出股四头肌。差速离心提取骨骼肌线粒体,分光光度法测定线粒体呼吸链酶复合体(CⅠ~CⅢ)活性。结果:(1)CⅠ活性:与NC组比较,QC组和QE组均显著下降(P<0.05,P<0.01);与NE组比较,QE组显著下降(P<0.05)。(2)CⅡ活性:与NC组比较,QC组显著升高(P<0.01);与QC组比较,QE组显著下降(P<0.01);与NE组比较,QE组显著上升(P<0.05)。(3)CⅢ活性:与NC组比较,QC组和NE组均显著上升(P<0.01)。结果提示:(1)单纯补充CoQ10和递增负荷训练均可提高力竭性运动后即刻骨骼肌线粒体呼吸链功能,且单纯补充CoQ10效果更佳。(2)递增负荷运动训练与补充CoQ10在提高力竭性运动后即刻骨骼肌线粒体功能方面无协同作用。
OBJECTIVE: To investigate the effects of exogenous CoQ10 (CoQ10) and incremental exercise training on the activity of mitochondrial respiratory chain complex Ⅰ, Ⅱ and Ⅲ in skeletal muscle of rats after exhaustive exercise. Methods: Thirty-six healthy 2-month-old male Wister rats were randomly divided into 4 groups (n = 9): control group (NC), CoQ10 supplemented group (QC), training group (NE) and supplemented CoQ10 + training group (QE). Feeding and training time for a total of 7 weeks. The rats in QC group and QE group were fed with CoQ10 once a day by 2mg / 100g body weight. NC group and QC group did not exercise training, NE group and QE group increased load level treadmill training. After 7 weeks, all the rats in each group were exhausted. After the exercise, the rats were decapitated and the quadriceps femoris was quickly removed. The skeletal muscle mitochondria were extracted by differential centrifugation and the mitochondrial respiratory chain enzyme complex (CⅠ-CⅢ) activity was measured by spectrophotometry. Results: (1) The activity of CⅠ: compared with NC group, the levels of QC and QE decreased significantly (P <0.05, P <0.01). Compared with NE group, QE decreased significantly (P <0.05). (2) Compared with NC group, QC group increased significantly (P <0.01), compared with QC group, QE group decreased significantly (P <0.01); compared with NE group, QE group increased significantly ). (3) CⅢ activity: Compared with NC group, QC group and NE group increased significantly (P <0.01). The results suggest that: (1) CoQ10 alone and incremental training can increase the respiratory chain function of skeletal muscle immediately after exhaustive exercise, and the supplement of CoQ10 is better. (2) Incremental Exercise Training and Supplementation CoQ10 has no synergistic effect in enhancing mitochondrial function of skeletal muscle immediately after exhaustive exercise.