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目的掌握甘肃口岸朝觐归国人员呼吸道病毒感染情况。方法 2014-2015年,采集朝觐归国人员中入境时体温升高(≥37.3℃)者的咽拭子样本,用荧光RT-PCR和荧光PCR方法检测流感病毒、副流感病毒、鼻病毒、冠状病毒、呼吸道合胞病毒、人偏肺病毒、腺病毒、人博卡病毒、中东呼吸综合征冠状病毒等共22种/型呼吸道病毒。结果在6 123名归国朝觐人员中,共发现337名体温升高者,采集337份咽拭子样本,检出流感病毒、鼻病毒、冠状病毒、人偏肺病毒等共51份,检测阳性率为15.13%。不同年龄和性别的病毒检测阳性率差异无统计学意义。结论初步了解甘肃朝觐人员呼吸道病毒病原谱构成,为保障朝觐人员健康工作提供参考。
Objective To know the respiratory virus infection of returning pilgrims from Gansu port. Methods From 2014 to 2015, throat swab samples from persons returning from pilgrimage to Hampi who returned to China with body temperature (≥37.3 ℃) were collected. Influenza virus, parainfluenza virus, rhinovirus, coronavirus were detected by fluorescence RT-PCR and fluorescence PCR Viruses, respiratory syncytial virus, human metapneumovirus, adenovirus, human Boka virus, Middle East respiratory syndrome coronavirus and a total of 22 kinds / type respiratory virus. Results A total of 337 persons with elevated body temperature were found among 6 123 returnees. 337 throat swab samples were collected and 51 samples of influenza virus, rhinovirus, coronavirus and human metapneumovirus were detected. Positive results were obtained The rate was 15.13%. There was no significant difference in the positive rates of virus detection between different ages and genders. Conclusions A preliminary understanding of the composition of the respiratory spectrum of respiratory virus among pilgrims in Gansu Province is provided for reference to ensure the health of pilgrims.