单纯形—紫外分光光度法测定复方维生素B注射液的含量

• 作 者: 邱家学 罗国安 程奇珍
• 机 构: 中国药科大学企业管理教研室,中国药科大学分析化学教研室,中国药科大学 1987届毕业生
• 出 处: 中国药科大学学报
• 发表日期: 1988年04期
• 关键词: 烟酰胺 单纯形法 紫外分光光度法 维生素B_6 四组分 摇匀 组分测定 平均相对误差 药品质量标准 原料药

采用紫外分光光度法测定复方维生素B注射液四组分的含量。在222～290nm区间内,间隔2nm测定吸光度,实验数据用单纯形法进行计算。维生素B_1、B_2、B_6和烟酰胺的平均回收率分别为100.0%,99.95%,100.1%和100.0%。样品的成分含量计算值与药品标准测定值的平均相对误差为1.28%。该法可用于多组分复方制剂的含量测定计算。

The contents of four components of compound vitamin B injection were determined by ultraviolet spectrophotometry. Absorbance was measured at intervals of 2 nm in the range of 222-290 nm. The experimental data were calculated by the simplex method. The average recoveries of vitamin B_1, B_2, B_6 and nicotinamide were 100.0%, 99.95%, 100.1% and 100.0%, respectively. The average relative error between the calculated value of the component of the sample and the measured value of the drug standard is 1.28%. The method can be used to calculate the content of multi-component compound preparations.