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目的探讨慢性持续性低氧对大鼠海马钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)表达量及其磷酸化水平的影响。方法选取清洁级SD成年雄性大鼠,随机分为6组每组6只。正常对照组(C组),低氧1d组(H1),低氧3d组(H3),低氧7d组(H7),低氧14d组(H14),低氧21d组(H21)。建立慢性持续性低氧模型。测量右心室收缩压,计算右心室肥厚指数,即右心室重量与左心室加室间隔重量比值[RV/(LV+S)]。断头取海马组织,应用10%SDS-PAGE和Western blot技术及GelDoc凝胶成像系统半定量检测T-CaMKⅡ和p-CaMKⅡ水平。结果与C组(100%)相比,H1[(77.6±14.9)%]、H3[(66.7±19.3)%]、H7[(79.6±21.7)%]、H14[(75.7±14.2)%]、H21[(78.8±12.9)%]组大鼠海马p-CaMKⅡ水平显著下降(P<0.05)。同时与C组(100%)相比,H1[(67.9±25.3)%]、H3[(74.1±23.2)%]、H7[(72.2±25.1)%]、H14[(75.3±12.4)%]、H21[(73.3±16.1)%]组大鼠海马CaMKⅡ蛋白表达量显著下降(P<0.05)。结论慢性持续性低氧可使大鼠海马组织内CaMKII活性下降,并抑制该蛋白的正常表达。
Objective To investigate the effect of chronic persistent hypoxia on the expression of calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) and its phosphorylation in hippocampus of rats. Methods SD male SD rats were randomly divided into six groups of 6 rats. Normal control group (C), hypoxia 1d group (H1), hypoxia 3d group (H3), hypoxia 7d group (H7), hypoxia 14d group (H14), hypoxia 21d group (H21). Establish a chronic persistent hypoxia model. Right ventricular systolic pressure was measured and the right ventricular hypertrophy index (RV / (LV + S)) was calculated as the weight ratio of right ventricle to left ventricular septum. The hippocampus tissues were decapitated and the levels of T-CaMKⅡ and p-CaMKⅡ were detected semi-quantitatively by using 10% SDS-PAGE, Western blot and GelDoc gel imaging system. Results H1 [(77.6 ± 14.9)%], H3 [(66.7 ± 19.3)%], H7 [(79.6 ± 21.7)%], H14 [(75.7 ± 14.2)%] , P21 (P <0.05) in hippocampus of H21 [(78.8 ± 12.9)%] group. H1 [(67.9 ± 25.3)%], H3 [(74.1 ± 23.2)%], H7 [(72.2 ± 25.1)%] and H14 [(75.3 ± 12.4)%] compared with group C (100% , And H21 [(73.3 ± 16.1)%] group, the expression of CaMKⅡprotein in hippocampus was significantly decreased (P <0.05). Conclusion Chronic persistent hypoxia can decrease the activity of CaMKII and inhibit the normal expression of CaMKII in hippocampus of rats.