论文部分内容阅读
目的探讨 Ras 相关结构域家族基因1A(RASSF1A)启动子区异常甲基化与食管癌发生发展的关系。方法采用甲基化特异性 PCR 方法,检测66例食管癌组织及相应的癌旁正常组织RASSF1A 基因启动子区异常甲基化。分析食管癌组织中 RASSF1A 基因启动子区异常甲基化与临床病理特征的关系。结果在食管癌组织、癌旁正常组织中 RASSF1A 基因启动子区异常甲基化的发生率分别为48.5%(32/66)、6.1%(4/66)。淋巴结转移者食管癌组织中 RASSF1A 基因启动子区异常甲基化的发生率(61.1%)明显高于无淋巴结转移者(33.3%)(x~2=5.055,P=0.025);晚期(Ⅲ-Ⅳ期)食管癌组织中 RASSF1A 基因启动子区异常甲基化的发生率(69.2%)明显高于早期(Ⅰ～Ⅱ期)(35.0%)(x~2=7.392,P=0.007);食管癌组织中 RASSF1A 基因启动子区异常甲基化与肿瘤分化程度无相关性(P>0.05)。结论食管癌组织中存在 RASSF1A 基因启动子区的异常甲基化,甲基化与食管癌病理分期和淋巴结转移有关。
Objective To investigate the relationship between aberrant methylation of Ras-related family member gene 1A (RASSF1A) promoter region and esophageal carcinogenesis. Methods Methylation-specific PCR was used to detect the abnormal methylation of RASSF1A promoter in 66 cases of esophageal cancer tissues and corresponding normal tissues. To analyze the relationship between methylation of RASSF1A promoter region and clinicopathological features in esophageal cancer. Results The rates of abnormal methylation of RASSF1A promoter region in esophageal cancer tissues and adjacent normal tissues were 48.5% (32/66) and 6.1% (4/66), respectively. The incidence of abnormal methylation of RASSF1A gene promoter region was significantly higher in patients with lymph node metastasis (61.1%) than in those without lymph node metastasis (33.3%) (x ~ 2 = 5.055, P = 0.025) Ⅳ). The incidence of abnormal methylation of RASSF1A gene promoter region (69.2%) in esophageal cancer was significantly higher than that in early stage (Ⅰ ~ Ⅱ) (35.0%) (x ~ 2 = 7.392, P = 0.007) There was no correlation between abnormal methylation of RASSF1A promoter region and tumor differentiation in cancer tissues (P> 0.05). Conclusion Abnormal methylation of RASSF1A gene promoter region exists in esophageal cancer tissues. Methylation is associated with pathological stage of esophageal cancer and lymph node metastasis.