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盐渍化是危害大豆生产的主要非生物胁迫因素之一。目前大豆耐盐性研究主要集中在栽培大豆的苗期耐盐性,而芽期耐盐性状的研究相对较少。野生大豆蕴含丰富的耐逆基因,是栽培大豆遗传改良的重要资源。为了研究野生大豆芽期耐盐性状的遗传机制,以113份野生大豆为试验材料,进行芽期耐盐性状的鉴定,结合群体的分子标记对包括2年平均值在内的3个环境下的3个耐盐指数进行全基因组关联分析,共检测到与野生大豆芽期耐盐相关的位点26个,6个SSR标记Satt521、Satt022、Satt239、Satt516、Satt251和Satt285在2个或3个环境下均被检测到,4个SSR标记Satt516、Satt251、Satt285和GMES4990与2个或3个耐盐指数显著相关。对这些SSR标记进行分析,挖掘了最优的等位基因及其载体材料。以上这些结果对于阐明野生大豆芽期耐盐性状的遗传机制,进一步发掘新的耐盐基因具有重要意义。同时也为栽培大豆遗传基础的拓宽、大豆耐盐分子标记辅助选择和分子设计育种等后续研究提供重要依据。
Salinization is one of the major abiotic stresses that endanger soybean production. At present, the research on salt tolerance of soybean mainly focuses on the salt tolerance of cultivated soybean at the seedling stage, while the research on salt tolerance at the bud stage is relatively few. Wild soybean is rich in gene resistance, is an important resource for genetic improvement of soybean cultivation. In order to study the genetic mechanism of salt-tolerant traits in wild soybean buds, 113 wild soybean cultivars were used to identify the salt tolerant traits at budding stage. The molecular markers were used to detect the salt tolerance in three environments, including the two-year average Totally 26 SSR markers Satt521, Satt022, Satt239, Satt516, Satt251 and Satt285 were detected in 2 or 3 environments The four SSR markers Satt516, Satt251, Satt285 and GMES4990 were significantly correlated with two or three salt tolerance index. The SSR markers were analyzed to find the best allele and its vector material. These results are of great significance for elucidating the genetic mechanism of salt tolerant traits in wild soybean and further exploring new salt tolerant genes. At the same time, it also provides an important basis for the follow-up research such as broadening the genetic basis of soybean cultivation, salt-tolerant molecular marker-assisted selection of soybean and molecular design and breeding.