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目的:探讨以葛根、灵芝、枳椇子、五味子、甘草为中药组方的口服液对酒精性肝损伤小鼠的保护功能及其作用机制。方法:建立小鼠酒精性肝损伤模型,测定并比较各组小鼠TP,ALT、AST、TG的水平与肝组织匀浆中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的含量和活性。结果:(1)与模型对照组相比,低、中、高3个剂量给药30 d后,小鼠血液中的乙醇含量均不同程度的降低,差异有统计学意义(P<0.05),且各剂量组具有较好的量效关系。(2)与模型对照组相比,低、中、高剂量组能够不同程度的改善小鼠血清肝功能生化指标,其中,中、高剂量组小鼠的AST、ALT活力明显下降,3个剂量组均明显降低血清TG含量,差异有统计学意义(P<0.01);高剂量组能明显降低肝脏中的MDA含量,差异有统计学意义(P<0.01),而低、中高剂量组,还均能够明显提升ADH和GSH-Px酶活力,差异有统计学意义(P<0.05)。结论:以葛根、灵芝、枳椇子、五味子、甘草为中药组方的口服液对小鼠酒精性肝损伤的有明确的保护功能,这对开发对酒精性肝损伤的有保护作用的药品与保健品方面具有一定的参考价值。
OBJECTIVE: To investigate the protective effect and its mechanism of Oral Liquid containing puerariae, Ganoderma lucidum, Hovenia dulcis Thunb, Schisandra chinensis and Glycyrrhiza uralensis on alcoholic liver injury in mice. Methods: The model of alcoholic liver injury in mice was established. The levels of TP, ALT, AST and TG in mice were measured and compared with those in liver homogenate. The levels of malondialdehyde (MDA), glutathione peroxidase (GSH) -Px) content and activity. Results: (1) Compared with the model control group, the ethanol content in the blood of mice decreased to some extent after 30 days of low, middle and high dose administration, the difference was statistically significant (P <0.05) And each dose group has a good dose-response relationship. (2) Compared with the model control group, the low, medium and high dose groups could improve the serum biochemical indexes of liver function in varying degrees. The AST and ALT activities of the middle and high dose groups were significantly decreased. The three doses (P <0.01); high dose group can significantly reduce the content of MDA in the liver, the difference was statistically significant (P <0.01), while the low, middle and high dose group All can significantly improve the activity of ADH and GSH-Px, the difference was statistically significant (P <0.05). Conclusion: Puerarin, Ganoderma lucidum, Hovenia Dulcis Thunb, Schisandra, licorice as the traditional Chinese medicine prescription oral liquid of mice with alcoholic liver injury have a clear protective function, which is developing alcoholic liver injury has protective effect of drugs and Health products have a certain reference value.