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采用改良的亨格特(Hungate)厌氧技术,用MPN法和滚管法同时测定水稻不同生育期土壤中的产甲烷菌数量有明显差异,在混合基质中生长的产甲烷菌数量,早稻在分蘖末期数量最高,可达3.6×10~(10)个/克干土;在H_2和CO_2生长的产甲烷菌数量,晚稻在分蘖盛期明显增高,到乳熟期可高达3.1×10~(11)个/克干土;在甲酸钠和乙酸钠基质中生长的产甲烷菌数量,晚稻在分蘖盛期较高,分别达3.7×10~8个/克千土和1.2×10~8个/克干土;在甲醇基质中生长的产甲烷菌数量,晚稻在各生育期差异不显著。不同深度土壤中产甲烷菌数量无显著差异。施用有机肥料在一定程度上可促进产甲烷菌数量的递增。水稻土中占优势的产甲烷菌种群为甲酸甲烷杆菌(Methanobacterium formicicum)、马氏甲烷八叠球菌(Methanosarcina mazei)和巴氏甲烷八叠球菌(Methanosarcina barkeri)。
Using the modified Hungate anaerobic technique, simultaneous determination of the numbers of methanogens in different growth stages of rice by MPN and tube rolling methods was significantly different. The number of methanogens grown in the mixed matrix, The highest number of tillering stage was up to 3.6 × 10 ~ (10) / g dry soil. The number of methanogens growing in H_2 and CO_2 increased significantly at tillering stage and up to 3.1 × 10 ~ 11) plants per gram of dry soil. The number of methanogens that grew in the sodium formate and sodium acetate substrates was higher at the tillering stage, reaching 3.7 × 10 ~ 8 grains / g soil and 1.2 × 10 ~ 8 grains / G dry soil; the number of methanogens that grow in the methanol matrix is not significantly different at different growth stages of late rice. There was no significant difference in the number of methanogens in soils at different depths. The application of organic fertilizer to a certain extent, can promote the increase of methanogens. Methanobacterium formicicum, Methanosarcina mazei and Methanosarcina barkeri are the dominant methanogenic bacteria in paddy soil.