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作者用PHA刺激人扁桃腺淋巴细胞培养制备粗IL—2,然后用饱和酸硫铵盐折,DEAE—Sepha dexA50离子交换层折,Seqhadex G100凝胶过滤三步法制备部分纯化的IL—2,用IL—2依赖细胞株MTHφ41.16测定IL—2活性单位。经部分纯化的IL—2活性达4800μ/ml,比活性6315,回收率54％,纯化倍数约200倍。作者应用部分纯化的IL—2诱导产生LAK细胞,对裸鼠实验性CNE——2Z肿瘤进行LAK/IL—2过继免疫治疗,获得明显疗效。肿瘤抑制率达84％(P<0.02)和70％(P<0.05)与对照动物比较,实验动物肿瘤生长潜伏期延长,肿瘤增殖速度缓慢,荷瘤率低。
The authors used PHA to stimulate human tonsillar lymphocyte culture to prepare crude IL-2, then use saturated ammonium thiosulfate, DEAE-Sephadex A50 ion exchange layer fold, and Seqhadex G100 gel filtration to prepare partially purified IL-2. The IL-2 activity unit was determined using the IL-2-dependent cell line MTHφ41.16. The partially purified IL-2 activity reached 4800 μ/ml, with a specific activity of 6315, a recovery rate of 54%, and a purification factor of about 200 times. The authors used partially purified IL-2 to induce LAK cells, and applied the LAK/IL-2 adoptive immunotherapy to experimental CNE-2Z tumors in nude mice, and obtained obvious curative effect. The tumor inhibition rate was 84% (P<0.02) and 70% (P<0.05). Compared with the control animals, the growth latency of the tumor in the experimental animals was prolonged, the tumor proliferation was slow, and the tumor-bearing rate was low.