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目的 建立一种新的鼻咽癌 (NPC)血清学诊断方法。方法 以纯化的EBV -DNase为抗原 ,采用WesternBlot法对NPC、头颈部其它肿瘤 (OT)及健康成人 (HA)血清中的EBV -DNase抗体进行检测。与同时进行的EBVCA -IgA抗体检测比较。 结果 ①用WesternBlot法检测NPC组、OT组、HA组血清中EBV -DNase抗体阳性率分别为 83.71% ,0 .0 0 % ,0 .0 0 % ,NPC组抗体阳性率与其它两组差异显著 (均为P <0 .0 1) ,后两组之间无显著差异 (P >0 .0 5)。而同时检测的EBVCA -IgA阳性率分别为85.98% ,9.38% ,7.50 %。两种方法阳性率比较 ,在NPC组无差异 (P >0 .0 5) ,而在其它两组的差异显著 (P <0 .0 5) ;②以病检结果为“金标准” ,EBV -DNase抗体WesternBlot法检测的特异性明显高于EBVCA-IgA检测 (P <0 .0 1) ,而两者的灵敏度相当 (P >0 .0 5)。结论 ①NPC血清中存在高滴度的EBV -DNase抗体 ;②用WesternBlot法检测血清中的EBV -DNase抗体 ,具有较高的灵敏度及特异性 ,可以减少NPC的漏诊和误诊 ,不失为一种NPC血清学诊断方法
Objective To establish a new method of serological diagnosis of nasopharyngeal carcinoma (NPC). Methods EBV-DNase antibodies in serum of NPC, other head and neck tumors (OT) and healthy adults (HA) were detected by Western Blot using purified EBV-DNase as antigen. Compared with simultaneous detection of EBVCA-IgA antibody. Results ① The positive rates of EBV-DNase antibody in serum of NPC, OT and HA groups were 83.71%, 0.00% and 0.00% respectively by Western blotting, and the positive rate of antibody in NPC group was significantly different from the other two groups (Both P <0.01), there was no significant difference between the two groups (P> 0.05). The positive rates of EBVCA-IgA detected at the same time were 85.98%, 9.38% and 7.50%, respectively. The positive rate of the two methods was no difference in NPC group (P> 0.05), while the difference between the other two groups was significant (P <0.05); ② The results of pathological examination were “gold standard”, EBV The specificity of -DNase antibody detected by WesternBlot was significantly higher than that of EBVCA-IgA assay (P <0.01), but the sensitivity of the two was similar (P> 0.05). Conclusion ① The high titer of EBV-DNase antibody in the serum of patients with NPC was found. ② The antibody of EBV-DNase in serum was detected by WesternBlot with high sensitivity and specificity, which could reduce the misdiagnosis and misdiagnosis of NPC as an NPC serology diagnosis method