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本文采用PCR -ELISA法检测 47例慢性乙型肝炎HBV基本核心启动子 (BCP)变异 ,同时使用OlympusAu 5 60自动生化仪检测患者肝功能 ,包括血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)、总胆红素 (TBi)、白蛋白 (ALB)及血清胆碱酯酶 (ChE)水平 ,用德国BE凝血仪检测患者血浆凝血酶原时间 (PT)、并根据公式计算出凝血酶原活动度(PTA)。结果示HBV -BCP变异阳性组患者较HBV -BCP变异阴性组ALT、AST、TBi略高 ,但无显著意义 (P >0 0 5 ) ,而ALB、ChE、PTA均明显降低 (P <0 .0 5 ) ,提示HBV -BCP变异阳性患者病情明显重于HBV -BCP变异阴性者。慢性乙型肝炎患者检测HBV -BCP变异对判断病情及预后有一定意义。
In this study, we detected the HBV core promoter (BCP) mutation in 47 patients with chronic hepatitis B by PCR-ELISA and detected the liver function, including serum ALT, AST, , Total bilirubin (TBi), albumin (ALB) and serum cholinesterase (ChE) were measured. Prothrombin time (PT) was measured by German BE blood coagulation analyzer and prothrombin activity was calculated according to the formula Degree (PTA). The results showed that HBV-BCP mutation positive group had slightly higher ALT, AST and TBi than HBV-BCP mutation negative group (P <0.05), but ALB, ChE and PTA were significantly lower (P <0. 0 5), suggesting that HBV-BCP mutation positive patients were significantly more severe than HBV-BCP mutation negative. Detection of HBV-BCP mutations in patients with chronic hepatitis B is of certain significance in judging the condition and prognosis.