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本研究采用ＡＢＣ免疫组化技术，对３０例食管鳞癌癌旁上皮内ＯＫＴ６阳性Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）和Ｓ－１００蛋白阳性ＬＣ进行了标记，着重观察了ＯＫＴ６阳性ＬＣ的分布及变化规律，发现从食管正常上皮，不典型增生上皮到原位癌，随着上皮分化程度的下降，ＯＫＴ６阳性ＬＣ数目逐渐减少，形态亦发生改变，提示ＯＫＴ６阳性ＬＣ与食管癌的发生有一定的关系。另外，ＯＫＴ６阳性ＬＣ在不同病变上皮内的分布明显不同，随着上皮分化程度的下降，ＯＫＴ６阳性ＬＣ群体逐渐上移至上皮的表层，这一结果为应用细胞学的方法检测ＬＣ以判定食管上皮的分化程度提供了另一线索。同时，我们还观察到，在食管正常上皮内，ＯＫＴ６阳性ＬＣ明显多于Ｓ－１００蛋白阳性ＬＣ，而在重增及原位癌时，ＯＫＴ６阳性ＬＣ明显少于Ｓ－１００蛋白阳性ＬＣ。结果表明，在食管粘膜中，存在着不同的ＬＣ亚群，ＯＫＴ６阳性ＬＣ比Ｓ－１００蛋白阳性ＬＣ对食管内环境的变化更为敏感。
In this study, ABC immunohistochemical technique was used to label OKT6-positive Langerhans cells (LC) and S-100 protein-positive LC in 30 cases of esophageal squamous cell carcinoma paraneoplastic epithelial cells. The distribution and variation of OKT6-positive LC were observed. From normal esophageal epithelium, atypical hyperplasia epithelium to carcinoma in situ, as the degree of epithelial differentiation decreased, the number of OKT6-positive LCs gradually decreased and the morphology also changed, suggesting that OKT6-positive LC has a certain relationship with the occurrence of esophageal cancer. In addition, the distribution of OKT6-positive LC in different epithelial lesions was significantly different. As the degree of epithelial differentiation decreased, OKT6-positive LC population gradually migrated to the epithelial surface. This result was used to detect LC by using cytology to determine the esophageal epithelium. The degree of differentiation provides another clue. At the same time, we also observed that in the normal esophageal epithelium, OKT6-positive LC was significantly more than S-100 protein-positive LC, whereas in re-increase and carcinoma in situ, OKT6-positive LC was significantly less than S-100 protein-positive LC. The results showed that there were different subgroups of LC in esophageal mucosa. OKT6-positive LC was more sensitive to changes in the esophageal environment than S-100 protein-positive LC.