论文部分内容阅读
通过显微观察和核糖体DNA转录间隔区(ITS)序列分析对引起乌鲁木齐地区三角荨麻白粉病的病原进行了鉴定。病原菌的分生孢子梗顶端产生一个椭圆形的分生孢子,分生孢子(18.75~23.75)μm×(31.25~43.75)μm;黑褐色、球形的闭囊壳嵌在叶表面的菌丝体中,直径为87.5~143.75μm;每个闭囊壳中有6或8个子囊,子囊无柄,大小为(25~43.75)μm×(37.5~68.75)μm,每个子囊中有4或5个子囊孢子,每个子囊孢子(18.75~21.88)μm×(25~31.25)μm。采用真菌ITS通用引物,扩增病菌r DNA ITS,测序及进化分析后发现,其ITS序列与伊朗异株荨麻白粉病菌(登录号AB104524)的同源性为99%,而且聚在一个进化支上。结果表明,乌鲁木齐三角荨麻白粉病菌属于Erysiphe urticae,与伊朗异株荨麻白粉病菌亲缘关系最近。
The pathogen causing triangle powdery mildew in Urumqi was identified by microscopic observation and ribosomal DNA transcribed spacer (ITS) sequence analysis. The top of the pathogen conidiophore produced an oval conidia, conidia (18.75 ~ 23.75) μm × (31.25 ~ 43.75) μm; dark brown, spherical closed shell embedded in the leaf surface mycelium , With a diameter of 87.5 ~ 143.75μm. There were 6 or 8 ascocycles in each capsule, with ascochondria sessile (25 ~ 43.75μm × (37.5 ~ 68.75) μm), 4 or 5 in each ascus Ascospores, each ascospore (18.75 ~ 21.88) μm × (25 ~ 31.25) μm. The ITS sequences of fungi were used to amplify the rDNA ITS of the bacteria. Sequencing and phylogenetic analysis showed that the ITS sequence shared 99% homology with B. urticaria (Accession No. AB104524) in Iran, and clustered in a clade on. The results showed that the Urumqi triangle net powdery mildew belongs to Erysiphe urticae, and the Iranian heterotrophic nettle powdery mildew closest genetic relationship.