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目的建立控制参附注射液中红参质量的HPLC指纹图谱。方法色谱柱为Discovery-C18(250mm×4.6mm,5μm),流动相为含2%四氢呋喃的乙腈溶液-水系统(梯度洗脱),检测波长203nm,柱温35℃,流速1.0mL·min-1,分析时间110min。结果在对照指纹图谱中确定了17个共有峰,测得10批参附注射液中红参皂苷指纹图谱相似度为0.992～0.997。结论所建方法重复性好,可为有效控制参附注射液的质量提供依据。
Objective To establish HPLC fingerprinting to control the quality of ginseng in Shenfu injection. Methods The mobile phase consisted of Discovery-C18 (250 mm × 4.6 mm, 5 μm) and mobile phase consisted of 2% tetrahydrofuran in acetonitrile (gradient elution). The detection wavelength was set at 203 nm and the column temperature was set at 35 ℃ with a flow rate of 1.0 mL · min- 1, analysis time 110min. Results Seventeen common peaks were identified in the control fingerprints. The similarity of the fingerprints of ginsenosides in 10 batches of Shenfu injection was 0.992-0.997. Conclusion The method has good repeatability and can provide the basis for effectively controlling the quality of Shenfu injection.